{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T22:27:27Z","timestamp":1791930447038,"version":"4.3.4"},"reference-count":81,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL158519"],"award-info":[{"award-number":["R01HL158519"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM066189"],"award-info":[{"award-number":["R01GM066189"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01DK113790"],"award-info":[{"award-number":["R01DK113790"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01HL094400"],"award-info":[{"award-number":["R01HL094400"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PJT\u2010178412"],"award-info":[{"award-number":["PJT\u2010178412"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    CD39 or NTPDase1 and other nucleoside triphosphate diphosphohydrolases (NTPDases), including NTPDase2, NTPDase3, and NTPDase8, regulate purinergic signaling through tuning the extracellular levels of purine nucleotides and nucleosides. Purinergic signaling regulates liver ischemia\u2013reperfusion (I\/R) injury, and CD39 is protective. However, the role of other NTPDases is unknown. In this study, we investigated the role of NTPDase2, NTPDase3, and NTPDase8. Global Entpd2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    , Entpd3\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    , and Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    and control wild type (WT) mice were subjected to liver I\/R. In addition, WT and Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice underwent global ischemia induced by hemorrhagic shock and resuscitation and injury evaluated. Bone marrow chimeric mice were generated to understand the role of NTPDase expression on hematopoietic cells in regulating liver injury. Although WT, Entpd2\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    and Entpd3\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice exhibited comparable levels of liver injury following local IR, Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice had increased liver injury compared to WT mice. Studies with bone marrow chimeric mice indicated that NTPDase8 on parenchymal liver cells protected against hepatic injury. This was confirmed by single\u2010nucleus RNAseq showing hepatocytes are the dominant cell type expressing NTPDase8 in the liver. Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice after I\/R injury were noted to have higher ATP concentrations in the liver and plasma. The P2 receptor antagonist suramin decreased liver injury in Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice indicating P2 signaling contributes to liver injury in these mice. Finally, Entpd8\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice had increased liver injury compared to WT mice also after hemorrhagic shock and resuscitation. These findings highlight the differential roles of NTPDase family members in the liver, with parenchymal\/hepatocyte expression of NTPDase8 emerging as a critical suppressor of the inflammatory and metabolic responses to hepatic I\/R insult, even in the presence of vascular NTPDase1 expression.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500010rr","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T10:41:17Z","timestamp":1751452877000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>NTPDase8<\/scp>\n                    Protects Against Liver Ischemia\u2013Reperfusion Injury in Mice"],"prefix":"10.1096","volume":"39","author":[{"given":"Taha","family":"Kelestemur","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Columbia University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon C.","family":"Robson","sequence":"additional","affiliation":[{"name":"Center for Inflammation Research, Department of Anesthesia and Division of Gastroenterology Beth Israel Deaconess Medical Center  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zolt\u00e1n H.","family":"N\u00e9meth","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Columbia University  New York New York USA"},{"name":"Department of Surgery Morristown Medical Center  Morristown New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pal","family":"Pacher","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Tissue Injury National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2261-0881","authenticated-orcid":false,"given":"Muhammed","family":"Arif","sequence":"additional","affiliation":[{"name":"Laboratory of Cardiovascular Physiology and Tissue Injury National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet S.","family":"Aydin","sequence":"additional","affiliation":[{"name":"Department of Histology &amp; Embryology, Faculty of Medicine Istanbul Medipol University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baris E.","family":"Suzek","sequence":"additional","affiliation":[{"name":"Department of Computer Engineering Mu\u011fla S\u0131tk\u0131 Kocman University  Mu\u011fla Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Pelletier","sequence":"additional","affiliation":[{"name":"Axe Maladies Infectieuses et Immunitaires CHU de Qu\u00e9bec \u2010 Universit\u00e9 Laval  Quebec City Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keri E.","family":"Lunsford","sequence":"additional","affiliation":[{"name":"Department of Surgery and Center for Immunity and Inflammation Rutgers New Jersey Medical School  Newark New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Schwabe","sequence":"additional","affiliation":[{"name":"Department of Medicine and Institute of Human Nutrition Columbia University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yana","family":"Geng","sequence":"additional","affiliation":[{"name":"Department of Medicine and Institute of Human Nutrition Columbia University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"S\u00e9vigny","sequence":"additional","affiliation":[{"name":"Axe Maladies Infectieuses et Immunitaires CHU de Qu\u00e9bec \u2010 Universit\u00e9 Laval  Quebec City Quebec Canada"},{"name":"D\u00e9partement de Microbiologie\u2010Infectiologie et D'immunologie, Centres PROTEO et ARThrite, Facult\u00e9 de M\u00e9decine Universit\u00e9 Laval  Quebec city Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gy\u00f6rgy","family":"Hask\u00f3","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Columbia University  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"issue":"2","key":"e_1_2_8_2_1","first-page":"78","article-title":"Inflammatory Mediators of Liver Ischemia\u2010Reperfusion Injury","volume":"7","author":"Walsh K. B.","year":"2009","journal-title":"Experimental and Clinical Transplantation"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707582"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2012.12.028"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-011-9239-6"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.11.006"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0402-358"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-006-9003-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2008.01.024"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.51.33116"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.8.2801"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00293.2006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2005.08.086"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2001342"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-021-09819-1"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0362222"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00293.2006"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000002230"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1998.5317"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00066-1"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01462.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702805"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(98)00063-6"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-005-6314-x"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2004.02.012"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0362222"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-017-9590-3"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2021.154731"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2021.114419"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.014878"},{"key":"e_1_2_8_31_1","first-page":"290","article-title":"P2 Purinoceptors in the Immune System","volume":"198","author":"Di Virgilio F.","year":"1996","journal-title":"Ciba Foundation Symposium"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.10.4634"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.99-0508com"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-107458"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-065870"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.11-190934"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.9.5616"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.2337\/db13-0573"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901295"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101224"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703134"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0702689"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2015.10"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2019.02.011"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3613"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2013.03.005"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-272450"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003379"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.14-253567"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.10.005"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9948"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3580001"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901851"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.36823"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1309468110"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjx058"},{"key":"e_1_2_8_57_1","article-title":"Murine Model of Metastatic Liver Tumors in the Setting of Ischemia Reperfusion Injury","volume":"150","author":"Yazdani H. O.","year":"2019","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.104.069658"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000001985"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0439-x"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2024.101101"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105330"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-023-00928-w"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-05289-6"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-023-02486-3"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e3181e14cd2"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2019.112338"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21175998"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00019.2010"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00126.2010"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-023-03315-6"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00115"},{"key":"e_1_2_8_79_1","first-page":"6060","volume-title":"Encyclopedia of Signaling Molecules","author":"Choi S.","year":"2017"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.8.5640"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.3c04303"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-320937"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202500010RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:53:54Z","timestamp":1763124834000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202500010RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":81,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7,15]]}},"alternative-id":["10.1096\/fj.202500010RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202500010rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]},"assertion":[{"value":"2025-01-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70775"}}