{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:08:42Z","timestamp":1788793722434,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,4,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The acute splenic response triggers a surge of immune cells and pro\u2010inflammatory mediators, exacerbating secondary brain injury and worsening the outcome after intracerebral hemorrhage (ICH). Splenectomy confers benefits in experimental ischaemic stroke, but its effects on ICH are not known. We conducted a proof\u2010of\u2010concept study to test the hypothesis that a prior splenectomy would improve behavioral outcomes after ICH. Adult C57BL\/6N mice with collagenase\u2010induced ICH that had undergone splenectomy two weeks prior were compared with those with intact spleens. Motor function, haematoma size, cerebral oedema, intracerebral and peripheral blood neutrophil counts were evaluated. Splenectomised mice had lower neutrophil counts in peripheral blood and peri\u2010haematoma regions of the brain. They performed better on the modified Neurological Severity Score during the first week post\u2010ICH and for a shorter duration on rotarod and cylinder tests. Haematomas in non\u2010splenectomised animals showed expansion after the initial hemorrhage and were larger than in splenectomised animals, with more severe surrounding oedema and greater mass effect. This is the first report on the beneficial effects of splenectomy in experimental ICH. Further studies on the optimal timing and mode of splenic response ablation are justified to assess its translational potential.<\/jats:p>","DOI":"10.1096\/fj.202500191r","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T09:15:12Z","timestamp":1744967712000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Splenectomy Improves Outcome of Intracerebral Hemorrhage"],"prefix":"10.1096","volume":"39","author":[{"given":"Chun Hin","family":"Leung","sequence":"first","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine, LKS Faculty of Medicine The University of Hong Kong  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hei Tung","family":"Shek","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine, LKS Faculty of Medicine The University of Hong Kong  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine, LKS Faculty of Medicine The University of Hong Kong  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karrie M.","family":"Kiang","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine, LKS Faculty of Medicine The University of Hong Kong  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilberto Ka\u2010Kit","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Clinical Medicine, LKS Faculty of Medicine The University of Hong Kong  Hong Kong Hong Kong"},{"name":"The State Key Laboratory of Brain and Cognitive Sciences The University of Hong Kong  Hong Kong Hong Kong"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/strokeaha.122.037155"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2016.03.003"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-022-02593-w"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0366-6999.20122483"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2016.08.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X15607880"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700324r"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hest.2020.09.001"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.11.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570159X19666210316092225"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.120.032196"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-018-1239-9"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2017.09.022"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jcbfm.9600217"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01079"},{"issue":"6","key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1212\/CON.0000000000000672","article-title":"Intracerebral Hemorrhage","volume":"24","author":"Ziai W. 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