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These oligosaccharides interact with mammalian lectin galectin\u20103, mediating cell surface receptor dynamics as well as cell\u2013cell and cell\u2010extracellular matrix interactions. Our previous and recent studies suggest that GlcNAc, in conjunction with galectin\u20103, enhances muscle regeneration in\u00a0vitro. We have also demonstrated that intraperitoneal GlcNAc administration improves muscle strength in\n                    <jats:italic>mdx<\/jats:italic>\n                    mice, a murine model of Duchenne muscular dystrophy (DMD). Here, we show that oral administration of GlcNAc significantly improves the spontaneous locomotor activity of\n                    <jats:italic>mdx<\/jats:italic>\n                    mice. Daily treatment with United States Pharmacopeia\u2010grade GlcNAc at doses of 0.6, 1.2, 1.8, and 2.4\u2009g\/kg body weight for 35\u2009days significantly enhanced nocturnal spontaneous locomotor activity, with the 1.2\u2009g\/kg body weight dose (equivalent to 0.144\u2009g\/kg body weight in humans) reducing damages of extensor digitorum longus muscle by nearly 50%. Although consecutive forced exercises, specifically horizontal and downhill treadmill running, reduced GlcNAc\u2010mediated improvement, mice treated with 0.6 and 1.2\u2009g\/kg body weight still showed increased overall spontaneous locomotor activity under this condition, despite the lack of protection against repeated eccentric contraction\u2010induced injury. These findings suggest that GlcNAc enhances overall muscle health, possibly through mechanisms other than direct protection from muscle injury. One possible contributing mechanism may involve enhanced muscle repair or regeneration, as suggested by our related in\u00a0vitro myogenesis work. Notably, co\u2010administration of GlcNAc with prednisolone, a corticosteroid commonly prescribed for DMD patients, further enhanced spontaneous locomotor improvement in\n                    <jats:italic>mdx<\/jats:italic>\n                    mice compared to prednisolone alone. These findings suggest that GlcNAc has the potential to improve the clinical status of DMD patients, either as a monotherapy or in combination with corticosteroids.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500196r","type":"journal-article","created":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T10:19:59Z","timestamp":1757931599000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Improvement of Spontaneous Locomotor Activity in a Murine Model of Duchenne Muscular Dystrophy by N\u2010Acetylglucosamine Alone and in Combination With Prednisolone"],"prefix":"10.1096","volume":"39","author":[{"given":"Masahiko. S.","family":"Satoh","sequence":"first","affiliation":[{"name":"Laboratory of DNA Damage Responses and Bioimaging, Research Centre of CHU de Quebec and Axis of Oncology, Faculty of Medicine Laval University  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillaume","family":"St\u2010Pierre","sequence":"additional","affiliation":[{"name":"Glycobiology and Bioimaging Laboratory of Research Center for Infectious Diseases and Axis of Infectious and Immunological Diseases, Research Centre of CHU de Quebec, Faculty of Medicine Laval University  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Rancourt","sequence":"additional","affiliation":[{"name":"Laboratory of DNA Damage Responses and Bioimaging, Research Centre of CHU de Quebec and Axis of Oncology, Faculty of Medicine Laval University  Quebec Canada"},{"name":"Glycobiology and Bioimaging Laboratory of Research Center for Infectious Diseases and Axis of Infectious and Immunological Diseases, Research Centre of CHU de Quebec, Faculty of Medicine Laval University  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maude","family":"Fillion","sequence":"additional","affiliation":[{"name":"Glycobiology and Bioimaging Laboratory of Research Center for Infectious Diseases and Axis of Infectious and Immunological Diseases, Research Centre of CHU de Quebec, Faculty of Medicine Laval University  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5960-1703","authenticated-orcid":false,"given":"Sachiko","family":"Sato","sequence":"additional","affiliation":[{"name":"Glycobiology and Bioimaging Laboratory of Research Center for Infectious Diseases and Axis of Infectious and Immunological Diseases, Research Centre of CHU de Quebec, Faculty of Medicine Laval University  Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010021\u201000248\u20103"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573\u2010023\u201000775\u20106"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(87)90579\u20104"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3460"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/355696a0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(88)90383\u20102"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/345315a0"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.6.1685"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1605265113"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1212\/wnl.0000000000002337"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/14740338.2024.2394578"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2022.2480"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701151rrr"},{"key":"e_1_2_9_15_1","first-page":"103","volume-title":"Essentials of Glycobiology","author":"Stanley P.","year":"2022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2024.107471"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.049"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m701890200"},{"key":"e_1_2_9_19_1","volume-title":"Galectins, Essentials of Glycobiology","author":"Cummings R. 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