{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:39:19Z","timestamp":1790786359439,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82170762"],"award-info":[{"award-number":["82170762"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000727"],"award-info":[{"award-number":["82000727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Peritoneal fibrosis is one of the main causes of peritoneal dysfunction and withdrawal of patients from peritoneal dialysis (PD). Our previous study has shown that high\u2010glucose dialysis solution promotes glycolysis in peritoneal mesothelial cells, leading to mesothelial\u2010to\u2010mesenchymal transition (MMT) and peritoneal fibrosis. However, the molecular mechanisms remain unclear. Using single\u2010cell RNA sequencing (scRNA\u2010seq) analysis of cells from the effluent of patients undergoing PD from our previous study, we found that pyruvate kinase isozymes M2 (PKM2), a key rate\u2010limiting enzyme of glucose metabolism, was significantly upregulated in the mesothelial cells of patients with long\u2010term peritoneal dialysis (LPD). Using co\u2010immunoprecipitation, chromatin immunoprecipitation assay, and gene silencing techniques, we revealed that PKM2 promotes the expression of transcription factor SNAI2 by acetylating histone H3K9 (H3K9ac), thereby promoting the occurrence of MMT and hence peritoneal fibrosis.\n                    <jats:sc>l<\/jats:sc>\n                    \u2010cysteine, a known PKM2 inhibitor, blocked these responses and prevented PD\u2010induced peritoneal fibrosis. These results could provide a novel therapeutic strategy for treating peritoneal fibrosis.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500229r","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T07:27:56Z","timestamp":1749626876000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>l<\/scp>\n                    \u2010Cysteine Alleviates Peritoneal Fibrosis by Repressing\n                    <scp>PKM2<\/scp>\n                    in Peritoneal Mesothelial Cells"],"prefix":"10.1096","volume":"39","author":[{"given":"Xiaokun","family":"Ma","sequence":"first","affiliation":[{"name":"Nephrology Division, Department of Medicine The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou China"},{"name":"Oncology Division, Department of Medicine The Third Affiliated Hospital of Sun Yat\u2010Sen University  Guangzhou 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