{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:39:35Z","timestamp":1790786375210,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T00:00:00Z","timestamp":1750982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100015508","name":"Shandong Academy of Medical Sciences","doi-asserted-by":"publisher","award":["202201\u2010062"],"award-info":[{"award-number":["202201\u2010062"]}],"id":[{"id":"10.13039\/501100015508","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The transcription factor PU.1 has been implicated in various fibrotic diseases; however, its role in peritoneal dialysis\u2010associated peritoneal fibrosis (PF) remains unclear. The role of PU.1 was investigated using knockdown approaches with AAV9\u2010shPU.1 in a rat PF model and siRNA in human peritoneal mesothelial cells (MeT\u20105A). Multiple experimental techniques, including real\u2010time quantitative PCR, western blot, enzyme\u2010linked immunosorbent assay (ELISA), and immunohistochemical staining, were utilized. In\u00a0vivo, PU.1 expression was significantly upregulated in the rat PF model. Knockdown of PU.1 ameliorated peritoneal thickening, collagen deposition, angiogenesis, inflammation, and oxidative stress. In\u00a0vitro, high\u2010glucose exposure upregulated PU.1 expression in MeT\u20105A cells, promoting fibrosis and angiogenesis. PU.1 knockdown reduced fibrosis\u2010related markers and suppressed the IL\u20106\/JAK1\/STAT3 signaling pathway, a critical regulator of PF. Treatment with an IL\u20106 inhibitor further confirmed that PU.1 modulates fibrosis through this pathway. PU.1 plays a pivotal role in the progression of PF. Its knockdown mitigates fibrosis by reducing inflammation and angiogenesis, and suppressing the activation of the IL\u20106\/JAK1\/STAT3 pathway, highlighting its potential as a therapeutic target.<\/jats:p>","DOI":"10.1096\/fj.202500305rr","type":"journal-article","created":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T11:51:52Z","timestamp":1751025112000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring\n                    <scp>PU<\/scp>\n                    .1 as a Therapeutic Target to Mitigate Oxidative Stress and Inflammation in Peritoneal Dialysis\u2010Induced Peritoneal Fibrosis"],"prefix":"10.1096","volume":"39","author":[{"given":"Shuo","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyan","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Medical Engineering Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Yanzhou District People's Hospital  Jining Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Nephrology The People's Hospital of Zouping City  Binzhou Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangzhou","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6932-8675","authenticated-orcid":false,"given":"Jie","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3828-6881","authenticated-orcid":false,"given":"Haiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shandong Provincial Hospital Affiliated to Shandong First Medical University  Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Nephrology Qilu Hospital of Shandong University  Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra2100152"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115246"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23094831"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.12.023"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-022-00834-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1498"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.84921"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-017-0571-2"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05587-5"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25168607"},{"issue":"3","key":"e_1_2_9_12_1","first-page":"1473","article-title":"Rapamycin Inhibits Peritoneal Fibrosis by Modifying Lipid Homeostasis in the Peritoneum","volume":"11","author":"Liu J.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00052.2017"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2004.00935.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-011-0147-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.104"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0896-x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16678"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2020.02.025"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.202309225"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20170926"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjv042"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.973182"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2018.11.004"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0396-0"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2020.4763"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006259"},{"issue":"3","key":"e_1_2_9_28_1","first-page":"3753","article-title":"Transcription Factor PU.1 Is Involved in the Progression of Glioma","volume":"15","author":"Xu Y.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110269"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13009"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-024-05398-y"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1080\/02648725.2023.2203002"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1106339"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0184332"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.5527\/wjn.v6.i3.111"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1177\/08968608231196033"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1797.2008.01062.x"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3109\/0886022X.2016.1165053"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.48064"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2369-14-185"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2369-15-8"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.122.001138"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/513015"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00396.2021"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2018.8"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00428.2016"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2015101169"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202500305RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:53:40Z","timestamp":1763124820000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202500305RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,27]]},"references-count":46,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7,15]]}},"alternative-id":["10.1096\/fj.202500305RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202500305rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,27]]},"assertion":[{"value":"2025-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70766"}}