{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T14:59:07Z","timestamp":1772204347270,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T00:00:00Z","timestamp":1751673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772405"],"award-info":[{"award-number":["81772405"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572100"],"award-info":[{"award-number":["81572100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Osteoporosis is one of the major bone disorders that has a close relationship with osteogenesis and angiogenesis. MiRNA coordinates a cascade of anabolic and catabolic processes in bone homeostasis and dynamic vascularization. Here, the role of miR\u201030d\u20105p in osteoporosis and its mechanism were investigated. To explore the effect of miR\u201030d\u20105p on osteogenesis and angiogenesis, an ovariectomized (OVX) mouse model, bioinformatics analysis, and multiple in\u00a0vitro assays were used. Compared to the sham group, OVX mice reduced BV\/TV, Tb.Th, Tb.N, and BMD, while it increased Tb.Sp. The number of osteoblasts and the density of microvessels were decreased in the OVX group, while the number of osteoclasts was increased. MiRNA sequencing and qRT\u2010PCR revealed that miR\u201030d\u20105p was increased in the bone marrow cells of the OVX mice. Supplementing miR\u201030d\u20105p activity using miR\u201030d\u20105p agomir inhibited osteogenic differentiation in bone marrow\u2010derived cells and prevented angiogenesis in HUVECs. Conversely, miR\u201030d\u20105p antagomir, an inhibitor of endogenous miR\u201030d\u20105p, reversed the inhibitory responses. Glucose regulating protein 78 (GRP78), a key regulator of endoplasmic reticulum stress, was demonstrated to be directly targeted by miR\u201030d\u20105p. Consistently, the overexpression of GRP78 improved miR\u201030d\u20105p\u2010related osteoblast differentiation and angiogenesis. We concluded that miR\u201030d\u20105p significantly inhibited bone formation and blood vessel formation by targeting GRP78 in experiments in\u00a0vitro. Therefore, miR\u201030d\u20105p may serve as a potential target for reversing osteoporosis.<\/jats:p>","DOI":"10.1096\/fj.202500345r","type":"journal-article","created":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T05:55:38Z","timestamp":1751694938000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>MiR<\/scp>\n                    \u201030d\u20105p Regulates Bone Remodeling and Vessel Remodeling in Osteoporosis by Targeting\n                    <scp>GRP78<\/scp>"],"prefix":"10.1096","volume":"39","author":[{"given":"Jie","family":"Li","sequence":"first","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ran","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang","family":"Li","sequence":"additional","affiliation":[{"name":"Tianjin Hospital, Tianjin University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinle","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidong","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianxiong","family":"Ma","sequence":"additional","affiliation":[{"name":"Tianjin Hospital, Tianjin University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Health Sciences and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering Hebei University of Technology  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Human Anatomy, School of Basic Medical Sciences Tianjin Medical University  Tianjin China"},{"name":"Key Laboratory of Hormones and Development (Ministry of Health), Tianjin Key Laboratory of Metabolic Diseases Tianjin Medical University  Tianjin China"},{"name":"Center for Health Sciences and Engineering, Hebei Key Laboratory of Biomaterials and Smart Theranostics, School of Health Sciences and Biomedical Engineering Hebei University of Technology  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-020-0339-7"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116258"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25042117"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00371.2018"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.8267"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202001080RR"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12050737"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.608521"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-021-00958-6"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2019.03.022"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom13071050"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201801851R"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2016.12.009"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2159-z"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.17450"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118317"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2017.03.032"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0000000000001454"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-03264-5"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052451"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt333"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2013.12.023"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-022-00976-y"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2021.108769"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-021-01201-8"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-021-2487-y"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp427"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.05005"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258643"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-0207-x"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201802711R"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.34818"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2021.12.011"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2022.116346"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05488-6"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242417319"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.2174\/0122115366266024240101075745"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.14760"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.42982"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48579-5"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2377-4"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1428-1"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2020.05.045"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12830"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02545-w"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/03008207.2018.1439485"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.118749"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.03.136"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.12.025"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2021.735622"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/2211-5463.13025"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-021-03775-3"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202500345R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:53:36Z","timestamp":1763124816000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202500345R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,5]]},"references-count":52,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7,15]]}},"alternative-id":["10.1096\/fj.202500345R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202500345r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,5]]},"assertion":[{"value":"2025-02-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70781"}}