{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:10:14Z","timestamp":1790604614397,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T00:00:00Z","timestamp":1749686400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100017950","name":"Shanghai Municipal Health Commission","doi-asserted-by":"publisher","award":["20214Y0331"],"award-info":[{"award-number":["20214Y0331"]}],"id":[{"id":"10.13039\/100017950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019978","name":"Shanghai Pudong New Area Health Commission","doi-asserted-by":"publisher","award":["PW2022A\u201068"],"award-info":[{"award-number":["PW2022A\u201068"]}],"id":[{"id":"10.13039\/501100019978","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,6,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Gestational diabetes mellitus (GDM) poses both short\u2010term and long\u2010term health risks to mothers and infants. This study aims to examine the mechanisms involving insulin resistance mediated by exosomal miRNAs. We enrolled 2284 pregnant women from First People's Hospital of Kunshan, Jiangsu Province in this continuous follow\u2010up study. Plasma exosomes from patients with GDM and healthy pregnancies during the first trimester were co\u2010cultured with human choriocarcinoma (JEG\u20103) cell lines. Plasma exosome samples collected at 8\u201312 and 24\u201328 gestational weeks were analyzed using high\u2010throughput small\u2010RNA sequencing. The expression levels of exosomal miRNAs were validated via real\u2010time polymerase chain reaction. Receiver operating characteristic (ROC) analysis was employed to assess their potential as early predictors. Plasma exosomes from the GDM group exhibited lower expressions of Let\u20107c\u20105p and miR\u201027a\u20105p compared to those from normal pregnancies by sequencing and validation during early pregnancy (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). These changes may influence the development and growth of JEG\u20103 cells. miR\u2010122\u20105p expression was elevated in GDM cases. The expression trajectories of these three miRNAs declined from early to late pregnancy. This miRNA panel showed potential as an early predictive biomarker for GDM (area under the ROC curve, 0.844;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Our findings demonstrate the significant role of exosomal miRNAs in GDM's pathogenesis and reveal a novel pattern of miRNA expression decline from early to late pregnancy. These miRNAs, particularly in combination, may be used as potential predictive biomarkers for GDM.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500470rr","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T06:05:43Z","timestamp":1749708343000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Exosome\n                    <scp>miRNA<\/scp>\n                    Profiles: The Reduced Expression of\n                    <scp>miRNA<\/scp>\n                    \u201027a\u20105p and Predictive\n                    <scp>miRNAs<\/scp>\n                    for Gestational Diabetes Mellitus"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6253-6626","authenticated-orcid":false,"given":"Yupei","family":"Shen","sequence":"first","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[{"name":"Changshou District Maternal and Child Health Hospital  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Li","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shanghai Pudong New Area Health Care Hospital for Women and Children  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwei","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"},{"name":"School of Pharmacy Xinxiang Medical University  Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianxi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Mao","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingjin","family":"Xia","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Difei","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6767-2763","authenticated-orcid":false,"given":"Jing","family":"Du","sequence":"additional","affiliation":[{"name":"Shanghai\u2010MOST Key Laboratory of Health and Disease Genomics, NHC Key Lab of Reproduction Regulation, Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-019-0098-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2019.05.001"},{"key":"e_1_2_11_4_1","article-title":"Gestational Diabetes Mellitus and Adverse Pregnancy Outcomes: Systematic Review and Meta\u2010Analysis","volume":"377","author":"Ye W.","year":"2022","journal-title":"BMJ (Clinical Research Ed.)"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.15659"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt013"},{"key":"e_1_2_11_7_1","doi-asserted-by":"crossref","unstructured":"T.Yahaya T.Salisu Y.Abdulrahman et\u00a0al. \u201cUpdate on the Genetic and Epigenetic Etiology of Gestational Diabetes Mellitus: A Review \u201d (2021).","DOI":"10.1186\/s43042-020-00054-8"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-022-00853-z"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2016.12.002"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/14791641221093901"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-0966"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2018-02693"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2021.02.012"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2022.864971"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2337\/db21-0909"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc09-1848"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-7498-6_8"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.6100"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-5164-1"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc16-0863"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-015-3811-5"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-2862"},{"key":"e_1_2_11_23_1","doi-asserted-by":"crossref","unstructured":"W.Lu M.Luo X.Fang R.Zhang andC.Hu \u201cDiscovery of Metabolic Biomarkers for Gestational Diabetes Mellitus in a Chinese Population \u201d2020.","DOI":"10.21203\/rs.3.rs-109665\/v1"},{"issue":"3","key":"e_1_2_11_24_1","first-page":"782","article-title":"Differential Expression Profile of Plasma Exosomal microRNAs in Women With Polycystic Ovary Syndrome \u2010 ScienceDirect","volume":"115","author":"XJaBC D.","year":"2020","journal-title":"Fertility and Sterility"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.08.006"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578-021-00533-4"},{"key":"e_1_2_11_27_1","doi-asserted-by":"crossref","unstructured":"C.Salomon K.Scholz\u2010Romero S.Sarkeret\u00a0al. \u201cGestational Diabetes Mellitus Is Associated With Changes in the Concentration and Bioactivity of Placenta\u2010Derived Exosomes in Maternal Circulation Across Gestation \u201d2016.","DOI":"10.2337\/db15-0966"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-018-0060-4"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201902522RR"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052572"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2019.02.016"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.2478\/acb-2020-0018"},{"issue":"5","key":"e_1_2_11_33_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0267564","article-title":"Circulating Extracellular Vesicles Exhibit a Differential miRNA Profile in Gestational Diabetes Mellitus Pregnancies","volume":"17","author":"Shanthie T.","year":"2022","journal-title":"PLoS One"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-022-00591-5"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1127336"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.22565"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102263"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808855115"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.fbe1502014"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/1753-0407.13450"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-02999-9"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/clinem\/dgad751"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202500470RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T14:52:09Z","timestamp":1763823129000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202500470RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,12]]},"references-count":41,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,6,30]]}},"alternative-id":["10.1096\/fj.202500470RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202500470rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,12]]},"assertion":[{"value":"2025-02-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70710"}}