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Here, we assess if human fecal microbiota collected in summer and winter and inoculated in the SHIME (simulating four different intestinal sections; ileum, and ascending, transverse and descending colon) and then treated with fish oil (FO) rich in triglyceride\u2010esterified docosahexaenoic and eicosapentaenoic acids (DHA and EPA, respectively) result in the formation of the corresponding anti\u2010inflammatory and anticancer n\u20103 PUFA metabolites, including\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acylethanolamines and oxylipins. Effluents were collected every day and analyzed by LC\u2013MS\/MS for PUFA metabolites and bacterial and short\u2010chain fatty acids (SCFA) composition during an initial 7\u2010day treatment with vehicle and then a further 7\u2010day treatment with FO (DHA and EPA in equal amounts, 910\u2009mg\/day each). A time\u2010dependent increase of DHA and EPA was observed, particularly with the summer microbiota in all intestinal sections except for the duodenum, which does not contain gut microbiota. The formation of 17\u2010hydroxy\u2010docosahexaenoic acid from DHA, and 15\u2010hydroxy\u2010 and 18\u2010hydroxy\u2010eicosapentaenoic acid from EPA was observed within the summer samples. However, no accumulation of\n                    <jats:italic>N<\/jats:italic>\n                    \u2010docosahexaenoyl\u2010ethanolamine or\n                    <jats:italic>N<\/jats:italic>\n                    \u2010eicosapentaenoyl\u2010ethanolamine, nor any other\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acyl\u2010ethanolamines, was detected in any intestinal section and season. These data suggest that a human fecal microbiome cultivated in a SHIME processes DHA and EPA\u2010containing triglycerides to oxylipins with known activity on the host, but not necessarily to\n                    <jats:italic>N<\/jats:italic>\n                    \u2010acyl\u2010ethanolamines.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500752rr","type":"journal-article","created":{"date-parts":[[2025,6,14]],"date-time":"2025-06-14T04:24:16Z","timestamp":1749875056000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Human Gut Microbes Produce EPA\u2010 and DHA\u2010Derived Oxylipins, but not\n                    <i>N<\/i>\n                    \u2010Acyl\u2010Ethanolamines, From Fish Oil"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7127-8152","authenticated-orcid":false,"given":"C.","family":"Roussel","sequence":"first","affiliation":[{"name":"Institute of Nutrition and Functional Foods (INAF), School of Nutrition Faculty of Agriculture and Food Sciences, Laval University  Quebec Quebec Canada"},{"name":"Centre Nutrition, Sant\u00e9 et Soci\u00e9t\u00e9 (NUTRISS) INAF Laval University  Quebec Quebec Canada"},{"name":"School of Pharmaceutical Sciences Faculty of Medicine, University of Ottawa  Ottawa Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lessard\u2010Lord","sequence":"additional","affiliation":[{"name":"Institute of Nutrition and Functional Foods (INAF), School of Nutrition Faculty of Agriculture and Food Sciences, Laval University  Quebec Quebec Canada"},{"name":"Centre Nutrition, Sant\u00e9 et Soci\u00e9t\u00e9 (NUTRISS) INAF Laval University  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Nallabelli","sequence":"additional","affiliation":[{"name":"Institute of Nutrition and Functional Foods (INAF), School of Nutrition Faculty of Agriculture and Food Sciences, Laval University  Quebec Quebec Canada"},{"name":"Heart and Lung University Research Center, Department of Medicine Faculty of Medicine, Laval University  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Muller","sequence":"additional","affiliation":[{"name":"Heart and Lung University Research Center, Department of Medicine Faculty of Medicine, Laval University  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5944-7200","authenticated-orcid":false,"given":"N.","family":"Flamand","sequence":"additional","affiliation":[{"name":"Heart and Lung University Research Center, Department of Medicine Faculty of Medicine, Laval University  Quebec Quebec 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