{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T04:42:57Z","timestamp":1784090577546,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T00:00:00Z","timestamp":1752105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Urinary tract infections (UTIs) are among the most common and persistent bacterial infections, affecting around 150 million people worldwide each year. The emergence of multidrug\u2010resistant (MDR) strains, particularly those producing extended\u2010spectrum \u03b2\u2010lactamases (ESBLs), has further complicated treatment and posed a serious global public health challenge. Hence, this study aimed to investigate the antibacterial potential of mesenchymal stem cell (MSCs) secretomes derived from adipose tissue (AD\u2010MSCs), bone marrow (BM\u2010MSCs), and umbilical cord (UC\u2010MSCs) against the most common cause of bacterial UTI,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). The MSCs secretomes were derived from human adipose tissue, umbilical cord, and bone marrow. The antibacterial activity of the secretomes was then assessed using the Kirby\u2010Bauer disk diffusion method. These tests were conducted on 105\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    urine clinical isolates, including ESBL\u2010producing strains. Further molecular analysis was performed to identify the resistant genes. Statistical analyses were then performed to test for statistically significant differences between MSCs sources. Our results showed that the stem cell secretome was effective against 63.8% of non\u2010ESBL and 52.7% of ESBL\u2010producing\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    isolates, with that derived from AD\u2010MSCs showing the largest inhibition zones. Molecular analysis revealed that all ESBL\u2010producing isolates (100%) harbored the CTX\u2010M gene either on its own or in combination with another gene. These results underscore the potential of MSC\u2010derived secretomes as a promising alternative for treating\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>E. coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    infections. Nonetheless, further research is required to explore their potential clinical uses as either complementary or standalone therapies for resistant infections.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500758r","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T07:24:37Z","timestamp":1752132277000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Therapeutic Effects of Mesenchymal Stem Cell Secretome Derived From Adipose Tissue, Umbilical Cord, and Bone Marrow Against Extended\u2010Spectrum Beta\u2010Lactamase\u2010Producing and Non\u2010Producing\n                    <scp>\n                      <i>Escherichia coli<\/i>\n                    <\/scp>\n                    Strains Isolated From Urinary Tract Infections"],"prefix":"10.1096","volume":"39","author":[{"given":"Leen","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences Beirut Arab University  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elie","family":"Salem Sokhn","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences Beirut Arab University  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charbel","family":"Khalil","sequence":"additional","affiliation":[{"name":"Reviva Regenerative Medicine Center  Bsalim Lebanon"},{"name":"Bone Marrow Transplant Unit, Burjeel Medical City  Abu Dhabi UAE"},{"name":"School of Medicine Lebanese American University  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3225-3673","authenticated-orcid":false,"given":"Fatima A.","family":"Saleh","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Technology, Faculty of Health Sciences Beirut Arab University  Beirut Lebanon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,10]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12040623"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2022.888205"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-06128-4"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.19592"},{"key":"e_1_2_13_6_1","unstructured":"Uroweb \u201cEAU Guidelines on Urological Infections \u201d[cited 2024 Dec 18] https:\/\/uroweb.org\/guidelines\/urological\u2010infections\/chapter\/the\u2010guideline."},{"key":"e_1_2_13_7_1","volume-title":"StatPearls","author":"Bono M. 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J.Perilla G.Ajello C.Bopp et\u00a0al. \u201cWHO_CDS_CSR_RMD_2003.6.pdf \u201d(2003) https:\/\/iris.who.int\/handle\/10665\/68554."},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-025-01695-w"},{"issue":"11","key":"e_1_2_13_39_1","first-page":"2937","article-title":"Extended\u2010Spectrum \u03b2\u2010Lactamases (ESBL): Challenges and Opportunities","volume":"11","author":"Husna A.","year":"2023","journal-title":"Biomedicine"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2024.05.004"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12522"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25061340"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00339"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2023.2234082"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202500758R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:53:39Z","timestamp":1763124819000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202500758R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,10]]},"references-count":43,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2025,7,15]]}},"alternative-id":["10.1096\/fj.202500758R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202500758r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,10]]},"assertion":[{"value":"2025-03-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70829"}}