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Human amniotic mesenchymal stem cells (hAMSC) have been reported as promising stem cells for clinical therapy due to their no tumorigenicity, low immunogenicity, no ethical concerns, potent proliferative potential, and tissue repair. At present, the effects and underlying mechanism of exosomes derived from hAMSC (hAMSC\u2010exo) on AGA are still not explored. Here, we reported that hAMSC\u2010exo accelerated hair growth in AGA mice. Then, single\u2010cell RNA sequencing was performed to analyze the cellular landscape of mouse dorsal skin, identifying 15 cell types and determining the differentiation trajectory of HF during hAMSC\u2010exo\u2010induced hair growth. It was found that hAMSC\u2010exo strengthened crosstalk between cycling HF cells and niche\u2010resident DPC, providing critical signaling for orchestrating hair follicle stem cells (HFSC) differentiation. Focusing on DPC, we further demonstrated that hAMSC\u2010exo alleviated DHT\u2010induced mitochondrial dysfunction and exerted protection against DHT\u2010induced DPC damage via Wnt\/\u03b2\u2010catenin signaling, which may reset the HFSC niche to a favorable environment for HF regeneration. These results revealed the protective effect and molecular mechanisms of hAMSC\u2010exo against hair loss at the single\u2010cell level and provided a novel therapy combating AGA.<\/jats:p>","DOI":"10.1096\/fj.202500853r","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T08:34:28Z","timestamp":1751877268000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Single\u2010Cell\n                    <scp>RNA<\/scp>\n                    Sequencing Highlights a Contribution of Human Amniotic Mesenchymal Stem Cells\u2010Derived Exosomes to Androgenetic Alopecia"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6010-9903","authenticated-orcid":false,"given":"Xiao\u2010Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Geriatrics Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College  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