{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T04:27:40Z","timestamp":1761712060838,"version":"build-2065373602"},"reference-count":46,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T00:00:00Z","timestamp":1752796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000090","name":"Congressionally Directed Medical Research Programs","doi-asserted-by":"publisher","award":["W81XWH\u201022\u20101\u20100317"],"award-info":[{"award-number":["W81XWH\u201022\u20101\u20100317"]}],"id":[{"id":"10.13039\/100000090","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P30GM145423"],"award-info":[{"award-number":["P30GM145423"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001298","name":"Presbyterian Health Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001298","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug resistance\u2010associated protein 1 (MRP1) is an ATP\u2010dependent transmembrane efflux pump that confers drug resistance. MRP1 is overexpressed in pancreatic ductal adenocarcinoma (PDAC) and other malignancies. However, its diagnostic value in cancer has not been explored. The present study examined MRP1 expression in plasma small extracellular vesicles (sEVs) derived from PDAC and colon cancer patients. The human plasma samples were obtained from the NCI\u2010sponsored Cooperative Human Tissue Network, Stephenson Cancer Center, and Oklahoma Blood Institute. sEVs were isolated using double filtration followed by the polymer precipitation method or using ultracentrifugation. Western blotting was performed to evaluate the expression level of MRP1, and proteomics were applied to confirm the detection of a short form of MRP1 in plasma sEVs. A short form of MRP1, representing the region between QCRL\u20101 and C\u2010terminus domains of the protein, was identified in plasma sEVs, and its level was significantly elevated in plasma sEVs derived from patients with early\u2010 and late\u2010stage PDAC, compared with matched healthy subjects (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200934 for each group). The level of the short form of MRP1 was also elevated in plasma sEVs derived from patients with colon cancer (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200944) but not in those derived from patients with breast cancer (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200915). The levels of the short form of MRP1 in plasma sEVs were not related to the chemotherapy status of the patients. We thus conclude that a short form of MRP1 is identified in plasma sEVs and its expression level is significantly elevated in patients with early\u2010stage and late\u2010stage PDAC, irrespective of their treatment status.\n                  <\/jats:p>","DOI":"10.1096\/fj.202500874r","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T09:05:17Z","timestamp":1752829517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Level of a Short Form of Multidrug Resistance\u2010Associated Protein 1 Is Elevated in Plasma Small Extracellular Vesicles Derived From Patients With Pancreatic Ductal Adenocarcinoma"],"prefix":"10.1096","volume":"39","author":[{"given":"Kritisha","family":"Bhandari","sequence":"first","affiliation":[{"name":"Department of Pathology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madison","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gillian","family":"Marshall","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeng Shi","family":"Kong","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Epidemiology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajay","family":"Jain","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred &amp; Pamela Buffett Cancer Center University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Hollingsworth","sequence":"additional","affiliation":[{"name":"Eppley Institute for Research in Cancer and Allied Diseases, Fred &amp; Pamela Buffett Cancer Center University of Nebraska Medical Center  Omaha Nebraska USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7358-3700","authenticated-orcid":false,"given":"Wei\u2010Qun","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Oklahoma College of Medicine  Oklahoma City Oklahoma USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21820"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.4708"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.01.259"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2020.188409"},{"key":"e_1_2_12_6_1","first-page":"105","article-title":"The Clinical Utility of Serum CA 19\u20109 in the Diagnosis, Prognosis and Management of Pancreatic Adenocarcinoma: An Evidence Based Appraisal","volume":"3","author":"Ballehaninna U. 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