{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:50:35Z","timestamp":1791280235933,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T00:00:00Z","timestamp":1760313600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bone marrow mesenchymal stem cells (BMSCs) can differentiate into osteoblasts and promote osteogenesis (OP), and the miRNAs carried by BMSCs\u2010derived exosomes (BMSC\u2010Exo) regulate osteoblast differentiation in bone\u2010related diseases. Additionally, abnormal mitochondrial autophagy in osteoblasts mediated by the SIRT1\/PINK1\/Parkin signaling axis affects differentiation and contributes to OP progression. Our preliminary study has demonstrated that miR\u201020b can target SIRT1 and osteogenic protein BMP2. The aim of the present study was to investigate the effect of BMSC\u2010Exo harboring miR\u201020b to mediate the SIRT1\/BMP2 in OP. Initially, through the successful isolation and characterization of BMSC\u2010Exo in\u00a0vitro, we co\u2010cultured these exosomes with osteoblasts and observed a significant enhancement in osteoblast differentiation, alkaline phosphatase activity, and mineral accumulation. Subsequent cell transfection experiments revealed that miR\u201020b\u2010overexpressed BMSC\u2010Exo further augmented osteogenic differentiation of osteoblasts, whereas interference with BMP2 expression attenuated this pro\u2010osteogenic effect. In a bilateral ovariectomised rat model of OP, we demonstrated that miR\u201020b\u2010overexpressed BMSC\u2010Exo treatment promoted bone formation and mitigated bone damage. Additionally, these exosomes restored mitochondrial membrane potential and autophagy, which were reversed by SIRT1 interference. Finally, osteoblasts isolated from OP model rats were used to further validate that miR\u201020b\u2010overexpressed BMSC\u2010Exo enhanced osteoblast differentiation and facilitated osteogenesis by activating SIRT1. These findings have collectively underscored the therapeutic potential of miR\u201020b\u2010enriched BMSC\u2010Exo in regard to OP treatment, providing a foundation for developing novel, targeted therapies for OP.<\/jats:p>","DOI":"10.1096\/fj.202501050rr","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:38:26Z","timestamp":1760405906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>MiR<\/scp>\n                    \u201020b\u2010Overexpressed\n                    <scp>BMSC<\/scp>\n                    \u2010Derived Exosomes Target\n                    <scp>SIRT1<\/scp>\n                    \/\n                    <scp>BMP2<\/scp>\n                    to Regulate Mitochondrial Autophagy and Osteogenesis in Osteoporosis"],"prefix":"10.1096","volume":"39","author":[{"given":"Zhe","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Affiliated Xiaoshan Hospital, Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Physical Examination Center Affiliated Xiaoshan Hospital, Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Nursing Affiliated Xiaoshan Hospital, Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Affiliated Xiaoshan Hospital, Hangzhou Normal University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Affiliated Xiaoshan Hospital, 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