{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T17:46:32Z","timestamp":1793814392675,"version":"4.6.1"},"reference-count":40,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014857","name":"National Natural Science Foundation of China-Guangdong Joint Fund","doi-asserted-by":"publisher","award":["32100009"],"award-info":[{"award-number":["32100009"]}],"id":[{"id":"10.13039\/501100014857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,8,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Evidences have demonstrated that the regulation of gut microbiota by probiotics can be utilized to prevent nonalcoholic fatty liver disease (NAFLD);\n                    <jats:italic>Bifidobacterium<\/jats:italic>\n                    was widely used as a potential therapeutic alternative for metabolic diseases. In this study, the probiotic functions of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bifidobacterium breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T and its effects on NAFLD were investigated through genome annotation and high\u2010fat diet (HFD)\u2010induced mouse model. The genome of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T consists of a single circular chromosome of 2\u2009372\u2009890\u2009bp. Eighteen male C57BL\/6J mice were fed a normal chow diet (NCD), a high\u2010fat diet, and a high\u2010fat diet (HFD) with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T separately for 12\u2009weeks. Histopathology, lipid content, biochemical markers in the liver and blood, and metagenomics were assessed and compared. Our results indicate that supplementation with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T alleviated liver damage, reduced fat accumulation in the liver and epididymal adipose tissue, decreased TNF\u2010\u03b1 concentration, and improved NAFLD activity scores. Metagenomic analysis revealed that administration of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T promoted a decreased Firmicutes\/Bacteroidetes ratio (F\/B), an increase in beneficial bacteria, and enhanced lipid metabolism functions. Correlation analysis between the differential species and NAFLD\u2010related indicators suggested that the anti\u2010NAFLD effect of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T is related to the gut microbiota. Therefore,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B. breve<\/jats:italic>\n                    <\/jats:styled-content>\n                    CM02\u201009T attenuates NAFLD by modulating the gut microbiota and could be used as an alternative therapeutic strategy.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501250rr","type":"journal-article","created":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T09:51:17Z","timestamp":1755683477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Ameliorating Effect of\n                    <i>Bifidobacterium breve<\/i>\n                    CM02\u201009T on High\u2010Fat Diet\u2010Induced Nonalcoholic Fatty Liver Disease in Mice"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9531-0057","authenticated-orcid":false,"given":"Yu","family":"Tian","sequence":"first","affiliation":[{"name":"College of Life Sciences Northwest University  Xi'an China"},{"name":"BGI Research  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianyue","family":"Xu","sequence":"additional","affiliation":[{"name":"BGI Research  Shenzhen China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhinan","family":"Wu","sequence":"additional","affiliation":[{"name":"BGI Research  Shenzhen China"},{"name":"College of Life Sciences University of Chinese Academy of Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyu","family":"Wang","sequence":"additional","affiliation":[{"name":"BGI Research  Shenzhen China"},{"name":"College 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