{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T16:53:03Z","timestamp":1793897583227,"version":"4.8.0"},"reference-count":72,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2021\u2010MS\u2010197"],"award-info":[{"award-number":["2021\u2010MS\u2010197"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012131","name":"Department of Science and Technology of Liaoning Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012131","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,8,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Disheveled binding antagonist of \u03b2\u2010catenin 3 (DACT3) is a negative regulator of canonical WNT signaling. Dysregulation of disheveled 1 (DVL1) plays a role in malignant progression. However, the mechanism by which DVL1 transmits signals in the canonical WNT pathway and the possible association between DVL1 and DACT3 remain unknown in non\u2010small cell lung cancer (NSCLC). Immunohistochemical evaluation of DACT3 and DVL1 expressions in resected NSCLC samples was performed. The biological behavior of NSCLC cells and the effect of transfection of DACT3\/DVL1 cDNA and siRNA\u2010DACT3 into NSCLC cells upon activation of canonical WNT signaling were examined, using Western blot, luciferase activity assay, immunofluorescence, and co\u2010immunoprecipitation. Reduced expression of DACT3 in NSCLC tissues showed a correlation with lymphatic metastasis and a poor prognosis in patients (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05). Additionally, a negative correlation was observed between the expression of DACT3 and that of DVL1. DACT3 inhibited the malignant phenotypes (invasion, proliferation, migration, tumorigenesis, and chemoresistance to cisplatin) of NSCLC cells induced by DVL1 through the WNT\/\u03b2\u2010catenin pathway. DACT3\u2010DVL1 interaction inhibited the phosphorylation of glycogen synthase kinase\u20103\u03b2 (GSK\u20103\u03b2) at serine 9 and \u03b2\u2010catenin at serine 675. Consequently, \u03b2\u2010catenin nuclear translocation was reduced, leading to the inactivation of \u03b2\u2010catenin\u2010mediated transcription and downregulating the expressions of the protein factors related to cell malignancy. Our study confirms that the interaction between DVL1 and DACT3 inhibits the DVL1\u2010induced activation of canonical WNT signaling for inhibiting the NSCLC progression and cisplatin resistance.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501462rr","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T10:02:57Z","timestamp":1755770577000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>DACT3<\/scp>\n                    \u2010\n                    <scp>DVL1<\/scp>\n                    Interaction\u2010Mediated Canonical\n                    <scp>WNT<\/scp>\n                    Signaling Regulates Non\u2010Small Cell Lung Cancer Progression and Cisplatin Resistance"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6681-3588","authenticated-orcid":false,"given":"Jingrong","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7981-0423","authenticated-orcid":false,"given":"Yudie","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2272-8754","authenticated-orcid":false,"given":"Mengdi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3557-3618","authenticated-orcid":false,"given":"Yang","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0863-1315","authenticated-orcid":false,"given":"Enhua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4041-5617","authenticated-orcid":false,"given":"Huanyu","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Hospital and College of Basic Medical Sciences China Medical University  Shenyang Liaoning People\u2019s Republic of 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