{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:50:29Z","timestamp":1791280229817,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Endometriosis (EMS) is a chronic gynecological disease. RND3 is recognized as a potential autophagy\u2010related biomarker in EMS. The aim of this study was to investigate the regulatory role of RND3 on autophagy and oxidative stress in EMS. Immunohistochemistry (IHC), RT\u2010qPCR, and western blot (WB) analyses were used to determine the expression levels of RND3 and PLEKHG5. The study assessed oxidative stress by examining NRF2\/NQO\u20101\/HO\u20101 expression, as well as GSH, SOD, MDA levels, and lipid ROS production. Autophagy was evaluated by analyzing the expression of autophagy\u2010related markers and phosphorylation of PI3K, AKT, ERK1\/2, and mTOR. Cell proliferation, migration, and invasion were evaluated using CCK\u20108 and Transwell assays. Apoptosis was assessed through flow cytometry, expression of apoptosis\u2010related markers, and TUNEL assay. The study also used ELISA to measure inflammatory factor levels and Co\u2010IP assay to investigate the interaction between RND3 and PLEKHG5. Low expression of RND3 was observed in both the eutopic and ectopic endometrial tissues and ectopic endometrial stromal cells (EESCs) from patients with EMS. Increasing RND3 levels reduced oxidative stress in EESCs, enhanced cellular autophagy, inhibited cell proliferation, migration, and invasion, and promoted apoptosis. Conversely, the knockdown of RND3 expression had the opposite effect. The impact of RND3 overexpression on oxidative stress, autophagy, and apoptosis in EESCs was reversed by si\u2010NRF2 and the autophagy inhibitor CQ. RND3 overexpression also upregulated the expression of PLEKHG5 in EESCs. Co\u2010IP results revealed an interaction between RND3 and PLEKHG5. In in\u00a0vivo experiments, low PLEKHG5 expression was observed in endometrial tissues of EMS mice, while RND3 overexpression alleviated EMS symptoms by decreasing oxidative stress and promoting cellular autophagy and apoptosis. RND3 inhibits EMS progression by enhancing autophagy and suppressing oxidative stress through PLEKHG5.<\/jats:p>","DOI":"10.1096\/fj.202501521rr","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:27:04Z","timestamp":1761391624000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>RND3<\/scp>\n                    Inhibits Endometriosis Progression by Regulating Autophagy and Oxidative Stress Through\n                    <scp>PLEKHG5<\/scp>"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2197-0332","authenticated-orcid":false,"given":"Han","family":"Li","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Xiangya Hospital of Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoshan","family":"Chai","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The Second Xiangya Hospital of Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2018.03.017"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.599117"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23031518"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcna.2022.10.010"},{"key":"e_1_2_10_6_1","first-page":"79","article-title":"Sexuality in Women With Endometriosis: A Critical Narrative Review","volume":"111","author":"La Rosa V. 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