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Toll\u2010like receptor 7 (TLR7), a sensor for single\u2010stranded RNA, plays a crucial role in resistance to viral infections, but its function during\n                    <jats:italic>T. gondii<\/jats:italic>\n                    infection remains unclear. In this study, we investigated the role and regulation of the TLR7\/MyD88\/IRF7 signaling pathway during\n                    <jats:italic>T. gondii<\/jats:italic>\n                    infection. RNA\u2010seq, qRT\u2010PCR, and Western blot analyses revealed significant downregulation of\n                    <jats:italic>Tlr7<\/jats:italic>\n                    expression in RAW264.7 cells at 24\u2009h post\u2010infection (hpi). Additionally, treatment with the TLR7 agonist imiquimod suppressed\n                    <jats:italic>T. gondii<\/jats:italic>\n                    proliferation in\u00a0vitro and prolonged survival in infected mice. Time\u2010course analysis uncovered a biphasic regulation of the TLR7\/MyD88\/IRF7 pathway: during the early infection phase (8\u2009hpi in\u00a0vitro or 12\u2009hpi in\u00a0vivo), these components were rapidly upregulated, along with a significant increase in\n                    <jats:italic>IFN\u2010\u03b2<\/jats:italic>\n                    mRNA levels. However, as the infection progressed (12\u201324\u2009hpi in\u00a0vitro or 24\u201396\u2009hpi in\u00a0vivo), the expression of these pathway components and IFN\u2010\u03b2 declined. Crucially, siRNA\u2010mediated knockdown of\n                    <jats:italic>Tlr7<\/jats:italic>\n                    abolished this biphasic regulation and significantly suppressed downstream\n                    <jats:italic>Irf7<\/jats:italic>\n                    and Ifnb1 expression, establishing TLR7 as essential for pathway activation and\n                    <jats:italic>IFN\u2010\u03b2<\/jats:italic>\n                    induction. This biphasic regulation of the pathway by\n                    <jats:italic>T. gondii<\/jats:italic>\n                    in RAW264.7 cells has not been previously reported. This study helps elucidate the role of Toll\u2010like receptors during\n                    <jats:italic>T. gondii<\/jats:italic>\n                    infection of the host and provides a new strategy for the treatment of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1096\/fj.202501808rr","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T14:45:17Z","timestamp":1764254717000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Biphasic Regulation of\n                    <scp>TLR7<\/scp>\n                    \/\n                    <scp>MyD88<\/scp>\n                    \/\n                    <scp>IRF7<\/scp>\n                    \u2010\n                    <scp>IFN<\/scp>\n                    \u2010\u03b2 Axis by\n                    <i>Toxoplasma gondii<\/i>\n                    Facilitates Immune Evasion"],"prefix":"10.1096","volume":"39","author":[{"given":"Xiaohua","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Parasitology, Xiangya School of Basic Medical Sciences Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Xiangya School of Basic Medical Sciences Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaijuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Xiangya School of Basic Medical Sciences Central South University  Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Xiangya School of Basic Medical Sciences Central South University  Changsha Hunan 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