{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:57:27Z","timestamp":1777946247542,"version":"3.51.4"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172912"],"award-info":[{"award-number":["32172912"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U23A20242"],"award-info":[{"award-number":["U23A20242"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["2023\u2010JCXK\u201001"],"award-info":[{"award-number":["2023\u2010JCXK\u201001"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,1,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rise of \u03b2\u2010lactamase\u2010producing bacteria remains a significant public health threat. Their rapid proliferation across various environments has led to a marked decline in the clinical effectiveness of \u03b2\u2010lactam antibiotics against these resistant strains. Targeting \u03b2\u2010lactamase activity offers a promising approach to managing resistant bacteria, especially methicillin\u2010resistant\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    (MRSA). Studies on in\u00a0vitro MIC killing curves, \u03b2\u2010lactamase activity assays, and quantitative real\u2010time PCR of related \u03b2\u2010lactamase genes demonstrate that 4\u2010methoxy salicyl aldehyde (HMB) unveiled its ability to significantly boost the potency of various \u03b2\u2010lactam antibiotics against the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    USA300 strain. Additionally, the study provided compelling evidence that HMB not only inhibits \u03b2\u2010lactamase activity but also amplifies the intracellular accumulation of reactive oxygen species within MRSA, thereby contributing to its heightened susceptibility to these antibiotics. It concurrently impacted both biofilm formation and the expression of related genes in MRSA. This dual mechanism of action underscores HMB's potential as a valuable adjunct in the treatment of \u03b2\u2010lactamase\u2010producing resistant bacteria. The combination of HMB and cefixime effectively protected host cells from MRSA\u2010induced damage in a co\u2010culture system. In\u00a0vivo experiments confirmed that the synergistic treatment effectively controlled\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    infections, which therapeutic strategy increased survival rates, reduced bacterial colonization, inflammation, and tissue damage in the pneumonia mouse model. Additionally, HMB significantly accelerated skin wound healing in those infected with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. aureus<\/jats:italic>\n                    <\/jats:styled-content>\n                    USA300. These findings identify HMB as a novel \u03b2\u2010lactamase inhibitor with the potential to combat \u03b2\u2010lactamase\u2010producing bacteria.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501828r","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T15:00:16Z","timestamp":1768230016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhancing the Susceptibility of Methicillin\u2010Resistant\n                    <i>Staphylococcus aureus<\/i>\n                    to \u03b2\u2010Lactam Antibiotics Through the Use of 4\u2010Methoxysalicyl Aldehyde"],"prefix":"10.1096","volume":"40","author":[{"given":"Jian","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery The First Hospital of Jilin University  Changchun China"},{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiayu","family":"Hou","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine Jilin University  Changchun 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