{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T01:58:06Z","timestamp":1777946286416,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,1,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To optimize the STAF score by incorporating NT\u2010proBNP and D\u2010dimer for improved prediction of cardioembolic stroke (CE) and to investigate their synergistic molecular mechanisms. A retrospective study enrolled 371 AIS patients classified by TOAST criteria (CE vs. non\u2010CE). Clinical, imaging, and biochemical data were used to construct\/evaluate a modified STAF. Diagnostic performance was assessed via ROC curves, DeLong's test, IDI, and NRI. In\u00a0vitro, H9c2 cardiomyocytes under hypoxia were treated with NT\u2010proBNP\/D\u2010dimer to assess viability, apoptosis, inflammation, and signaling. In\u00a0vivo, an AF rat model with cerebral embolism received AAV\u2010mediated interventions (overexpression\/knockdown). The modified STAF incorporated NT\u2010proBNP \u2265\u2009321.5\u2009ng\/L and D\u2010dimer \u2265\u20090.67\u2009\u03bcg\/mL, achieving a significantly higher AUC (0.942) than the original STAF (0.846,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). In\u00a0vitro, NT\u2010proBNP and D\u2010dimer synergistically increased apoptosis and inflammation in cardiomyocytes by activating NF\u2010\u03baB. In\u00a0vivo, overexpression exacerbated cerebral infarct size and upregulated NLRP3 pathway proteins, while knockdown mitigated these injuries. Incorporating NT\u2010proBNP and D\u2010dimer significantly enhances STAF accuracy for CE prediction. These biomarkers synergistically promote cardioembolic inflammation and injury via NF\u2010\u03baB\u2010NLRP3 pathway activation, suggesting potential therapeutic targets.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501912r","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T13:22:33Z","timestamp":1768224153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Insights Into Cardioembolism:\n                    <scp>NT<\/scp>\n                    \u2010\n                    <scp>proBNP<\/scp>\n                    and D\u2010Dimer Augment Risk Stratification and Drive\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    \/\n                    <scp>NLRP3<\/scp>\n                    \u2010Mediated Injury"],"prefix":"10.1096","volume":"40","author":[{"given":"Yuhang","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Neurology The Affiliated Wenling Hospital of Wenzhou Medical University (The First People's Hospital of Wenling)  Wenling China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5757-7367","authenticated-orcid":false,"given":"Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Neurology The Affiliated Wenling Hospital of Wenzhou Medical University (The First People's Hospital of Wenling)  Wenling China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2023.12.010"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.26867"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.025250"},{"key":"e_1_2_13_5_1","first-page":"35","article-title":"Risk Factors for and Incidence of Subtypes of Ischemic Stroke","volume":"27","author":"Ihle\u2010Hansen H.","year":"2012","journal-title":"Functional Neurology"},{"key":"e_1_2_13_6_1","doi-asserted-by":"crossref","unstructured":"L.Xu X.Yilong A.Chunxue andN.Yongjun \u201cTOAST Subtypes: Its Influence Upon Doctor Decisions of Antihypertensive Prescription at Discharge for Ischemic Stroke Patients 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