{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T16:43:18Z","timestamp":1792773798670,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T00:00:00Z","timestamp":1767139200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82103753"],"award-info":[{"award-number":["82103753"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Skin\u2010derived precursors (SKPs) isolated from the dermis are poorly immunogenic and capable of modulating the allogeneic immune responses. Previous studies implied mouse SKPs\u2010derived exosomes (mSKPs\u2010exo) might possess immunomodulatory functions. In the present study we collected mSKPs\u2010exo using ultracentrifugation method and characterized them. We then explored mSKPs\u2010exo's impact on acne inflammation with\n                    <jats:italic>Cutibacterium acnes<\/jats:italic>\n                    (\n                    <jats:italic>C. acnes<\/jats:italic>\n                    )\u2010challenged murine monocyte\u2010macrophages RAW264.7 and Sprague Dawley (SD) rats auricular acne model. The in\u00a0vitro studies demonstrated mSKPs\u2010exo reduced nitric oxide, TNF\u2010\u03b1, and IL\u20106 concentration in\n                    <jats:italic>C. acnes<\/jats:italic>\n                    \u2010challenged RAW264.7 cells supernatant. mSKPs\u2010exo also ameliorated NF\u2010\u03baB p65 nuclear translocation and CD86 and iNOS antigens expression in\n                    <jats:italic>C. acnes<\/jats:italic>\n                    \u2010challenged RAW264.7 cells. The RT\u2010PCR and Western blots revealed mSKPs\u2010exo alleviated inflammation by regulating key genes (TLR2, TNF\u2010\u03b1, MyD88, and IL\u20106) and proteins (TLR2, MyD88, I\u03baBa, and NF\u2010\u03baBp65) in the TLR2\/MyD88\/NF\u2010\u03baB pathway. The anti\u2010inflammatory effects could be synergistically enhanced by I\u03baB\/IKK inhibitor. The in\u00a0vivo studies validated mSKPs\u2010exo's efficacy on acne inflammation and TLR2\/MyD88\/NF\u2010\u03baB pathway. Topical dermal injection of mSKPs\u2010exo alleviated auricular inflammatory manifestations in SD rats. Immunohistochemistry and Western blot results indicated mSKPs\u2010exo reduced the expression of TLR2, MyD88, MMP3, p\u2010p38 MAPK, and NF\u2010\u03baB p65. The effect was comparable with topical adapalene gel application. The current study initiated mSKPs\u2010exo research and provided theoretical support and experimental data for their potential application in inflammation regulation, acne treatment, and novel therapeutic targets identification.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501944r","type":"journal-article","created":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T14:34:17Z","timestamp":1767191657000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Skin\u2010Derived Precursors\u2010Derived Exosomes Alleviated Acne Inflammation via\n                    <scp>TLR2<\/scp>\n                    \/\n                    <scp>MyD88<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    Signaling Pathway: In\u00a0Vitro and In\u00a0Vivo Studies"],"prefix":"10.1096","volume":"40","author":[{"given":"Yiming","family":"Li","sequence":"first","affiliation":[{"name":"Department of Dermatology Institute of Traditional Chinese Medicine of Sichuan Academy of Chinese Medicine Sciences  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyi","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory of Ethnopharmacology, Tissue\u2010Orientated Property of Chinese Medicine Key Laboratory of Sichuan Province, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manlin","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology Renmin Hospital of Wuhan University  Wuhan Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangqi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Clinical Research Center for Ocular Diseases State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat\u2010Sen University, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science  Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Dermatology Institute of Traditional Chinese Medicine of Sichuan Academy of Chinese Medicine Sciences  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Bo","sequence":"additional","affiliation":[{"name":"Department of Dermatology Institute of Traditional Chinese Medicine of Sichuan Academy of Chinese Medicine Sciences  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6815-2954","authenticated-orcid":false,"given":"Li","family":"Li","sequence":"additional","affiliation":[{"name":"Evaluation Center for Cosmetics Safety and Efficacy, West China Hospital Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,31]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2023.12.017"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-4642(22)00314-5"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2021.17633"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1692"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2023.11.016"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2024.101196"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.327353"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2020.1717042"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2024.112873"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2018.07.001"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2024.102769"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2024.122542"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/biol-2021-0128"},{"key":"e_1_2_13_15_1","first-page":"2803","article-title":"Isolation of Skin\u2010Derived Precursors (SKPs) and Differentiation and Enrichment of Their Schwann Cell Progeny","volume":"1","author":"Miller F. 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