{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:51:06Z","timestamp":1793659866384,"version":"4.5.1"},"reference-count":64,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009558","name":"University Natural Science Research Project of Anhui Province","doi-asserted-by":"publisher","award":["2023AH051969"],"award-info":[{"award-number":["2023AH051969"]}],"id":[{"id":"10.13039\/501100009558","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2025,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We pioneer a multimodal framework integrating single\u2010cell RNA sequencing (scRNA\u2010seq), radiomics, and deep learning to decipher dendritic cell (DC)\u2010mediated mechanisms underlying anti\u2010PD\u20101 response in non\u2010small cell lung cancer (NSCLC). Single\u2010cell RNA sequencing of tumor samples from responders and non\u2010responders identified nine immune cell types, among which DCs displayed significant differences between groups. Cell\u2013cell communication and pseudotime analyses highlighted conventional DC2 (cDC2) and tolerogenic DC (tDC) as key subsets linked to therapeutic outcomes. Four cDC2 marker genes (\n                    <jats:italic>FAM3B<\/jats:italic>\n                    ,\n                    <jats:italic>TFAP2A<\/jats:italic>\n                    ,\n                    <jats:italic>RTKN2<\/jats:italic>\n                    , and\n                    <jats:italic>XCL2<\/jats:italic>\n                    ) and two tDC marker genes (\n                    <jats:italic>KRT6A<\/jats:italic>\n                    and\n                    <jats:italic>RAB27B<\/jats:italic>\n                    ) showed predictive value, and experimental validation confirmed reduced cDC2 and tDC abundance in responders, with upregulation of\n                    <jats:italic>FAM3B<\/jats:italic>\n                    ,\n                    <jats:italic>RTKN2<\/jats:italic>\n                    , and\n                    <jats:italic>XCL2<\/jats:italic>\n                    , and downregulation of\n                    <jats:italic>TFAP2A<\/jats:italic>\n                    ,\n                    <jats:italic>KRT6A<\/jats:italic>\n                    , and\n                    <jats:italic>RAB27B<\/jats:italic>\n                    . DCs also modulated CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell activity via OSM\u2013IL6ST and IL15\u2013IL15RA signaling. These six genes were incorporated into machine learning models combining transcriptomic (LSTM), clinical (EnhancedClinicalRNA), and radiomic (ResNet50) data. A stacked ensemble learning approach integrating all three modalities achieved superior performance, with an accuracy of 0.97 and an AUC of 0.99. In summary, our results demonstrate that combining single\u2010cell transcriptomics and radiomics through ensemble deep learning enables accurate prediction of immunotherapy response in NSCLC, and identifies six DC\u2013associated marker genes with potential as prognostic biomarkers.\n                  <\/jats:p>","DOI":"10.1096\/fj.202501990r","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:51:14Z","timestamp":1761763874000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Decoding Dendritic Cell Subtypes via Integrated Radiogenomics: A Stacked Ensemble Model for Predicting Immunotherapy Response in\n                    <scp>NSCLC<\/scp>"],"prefix":"10.1096","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6258-7986","authenticated-orcid":false,"given":"Qiong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Science Anhui Medical University  Hefei China"},{"name":"Department of Infectious Diseases First Affiliated Hospital of Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine First Affiliated Hospital of Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyue","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongran","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yushu","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Jia","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqing","family":"Qian","sequence":"additional","affiliation":[{"name":"School of Basic Medicine Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine First Affiliated Hospital of Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meimei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Science Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuliang","family":"Chen","sequence":"additional","affiliation":[{"name":"Bengbu Medical University Joint Research Center for Regional Diseases of IHM  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2023.1308525"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/21655979.2021.2011639"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010022\u201009563\u20108"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.2356"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12359"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010023\u201001740\u2010y"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12327"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clon.2024.06.002"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010022\u201001618\u20105"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.783236"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010023\u201001924\u20106"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201005400\u2010x"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844142"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010pathmechdis\u2010031521\u2010041113"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2022.02.013"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.730"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010022\u201004219\u2010z"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.09.011"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2023.08.009"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.10.020"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.07.034"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588\u2010021\u201000911\u20101"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.01.010"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.694"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20101307\u20108_19"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045\u2010021\u201001105\u20102"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592\u2010021\u201001203\u20106"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010023\u201005795\u20101"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12162042"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2023.5555"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15184463"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.04.013"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13073\u2010021\u201000968\u2010x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2021.109717"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2023.07.006"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11538\u2010020\u201000851\u20107"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201020657\u20104"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchf.2022.05.010"},{"key":"e_1_2_11_40_1","doi-asserted-by":"crossref","unstructured":"A.Rafiei M. G.Rad A.Sikora andR.Kamaleswaran \u201cImproving Irregular Temporal Modeling by Integrating Synthetic Data to the Electronic Medical Record Using Conditional GANs: A Case Study of Fluid Overload Prediction in the Intensive Care Unit \u201dmedRxiv https:\/\/doi.org\/10.1101\/2023.06.20.23291680.","DOI":"10.1101\/2023.06.20.23291680"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.202109\u20102019oc"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/s2589\u20107500(22)00112\u20101"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20102962\u20108_10"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102024\u2010008983"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02829"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565\u2010021\u201009613\u20100"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052714199"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010023\u201002804\u2010z"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102021\u2010003213"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.09.001"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12151970"},{"key":"e_1_2_11_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010021\u201021246\u20109"},{"key":"e_1_2_11_53_1","doi-asserted-by":"publisher","DOI":"10.2340\/00015555\u20103505"},{"key":"e_1_2_11_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.7b00485"},{"key":"e_1_2_11_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201021389\u20109"},{"key":"e_1_2_11_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/ibd\/izad084"},{"key":"e_1_2_11_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12872\u2010023\u201003574\u20104"},{"key":"e_1_2_11_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241411297"},{"key":"e_1_2_11_59_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc\u20102022\u2010004892"},{"key":"e_1_2_11_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010022\u201005309\u20105"},{"key":"e_1_2_11_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2023.05.032"},{"key":"e_1_2_11_62_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1076883"},{"key":"e_1_2_11_63_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.3c01140"},{"key":"e_1_2_11_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.1069673"},{"key":"e_1_2_11_65_1","doi-asserted-by":"publisher","DOI":"10.2741\/4647"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202501990R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202501990R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202501990R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T16:02:57Z","timestamp":1771257777000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202501990R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":64,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2025,11,15]]}},"alternative-id":["10.1096\/fj.202501990R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202501990r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]},"assertion":[{"value":"2025-06-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71133"}}