{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:20:34Z","timestamp":1791415234251,"version":"4.3.3"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2021J011342"],"award-info":[{"award-number":["2021J011342"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010166","name":"Xiamen Municipal Bureau of Science and Technology","doi-asserted-by":"publisher","award":["3502Z20244ZD1002"],"award-info":[{"award-number":["3502Z20244ZD1002"]}],"id":[{"id":"10.13039\/100010166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005270","name":"Fujian Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["2021Y201020028"],"award-info":[{"award-number":["2021Y201020028"]}],"id":[{"id":"10.13039\/501100005270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470763"],"award-info":[{"award-number":["82470763"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,3,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The regulatory mechanisms underlying M1\/M2 macrophage polarization and efferocytosis in acute kidney injury (AKI) remain poorly understood. This study aimed to investigate the mechanism by which RBM3 modulates macrophage function to alleviate burn\u2010induced AKI. A standardized scald burn model was established in rats by dorsal immersion in 100\u00b0C water for 15\u2009s. Renal function and histopathology were evaluated at 24, 48, and 72\u2009h post\u2010injury through serum biochemistry and hematoxylin and eosin (H&amp;E) staining. RBM3 expression was analyzed by qRT\u2010PCR, Western blot, immunohistochemistry, and flow cytometry. Adenoviral RBM3 was administered to AKI rats, followed by a comprehensive assessment including renal function tests, H&amp;E\/TUNEL staining, KIM\u20101 immunofluorescence, macrophage polarization analysis (M1\/M2 markers), and cytokine profiling (IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1). Single\u2010cell RNA sequencing identified differentially expressed genes, particularly Slfn4, validated via in\u00a0vitro knockdown and dual RBM3\/Slfn4 interference experiments in bone marrow\u2010derived macrophages to assess polarization and efferocytosis. Peak AKI severity occurred at 48\u2009h post\u2010burn, coinciding with dynamic RBM3 expression. RBM3 overexpression significantly improved renal function, attenuated tissue damage, reduced inflammation as evidenced by decreased levels of IL\u20106, IL\u20101\u03b2, and TNF\u2010\u03b1, promoted M2 polarization characterized by increased Arg1 expression and decreased CD86 and iNOS levels, and enhanced efferocytosis. Slfn4 knockdown replicated the protective effects of RBM3, while RBM3 inhibition exacerbated M1 polarization, effects that were rescued by concurrent Slfn4 suppression. RBM3 ameliorates burn\u2010induced AKI by downregulating Slfn4 to drive a pro\u2010resolving macrophage program. The RBM3\u2010Slfn4 axis may represent a potential therapeutic target for AKI management.<\/jats:p>","DOI":"10.1096\/fj.202503604rr","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T16:01:04Z","timestamp":1773849664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Augmenting the Endogenous\n                    <scp>RBM3<\/scp>\n                    Response Protects Against Burn\u2010Induced Acute Kidney Injury by Enhancing Macrophage\u2010Mediated Resolution of Inflammation"],"prefix":"10.1096","volume":"40","author":[{"given":"Zhengjie","family":"Huang","sequence":"first","affiliation":[{"name":"The Graduate School of Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zihang","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofan","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuojin","family":"Yang","sequence":"additional","affiliation":[{"name":"The Graduate School of Fujian Medical University  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbin","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Xiamen Key Laboratory of Precision Diagnosis and Treatment of Chronic Kidney Disease The Fifth Hospital of Xiamen  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiping","family":"Hu","sequence":"additional","affiliation":[{"name":"The Graduate School of Fujian Medical University  Fuzhou Fujian China"},{"name":"Department of Nephrology, Fujian Clinical Research Center for Chronic Glomerular Disease The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8496-2087","authenticated-orcid":false,"given":"Jiyi","family":"Huang","sequence":"additional","affiliation":[{"name":"The Graduate School of Fujian Medical University  Fuzhou Fujian China"},{"name":"Department of Nephrology, Xiamen Key Laboratory of Precision Diagnosis and Treatment of Chronic Kidney Disease The Fifth Hospital of Xiamen  Xiamen Fujian China"},{"name":"Department of Nephrology, Fujian Clinical Research Center for Chronic Glomerular Disease The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)02385-7"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00284-z"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11193073"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00769-y"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2021.04.013"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2021.153818"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418-022-01033-9"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.81240"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-024-00875-5"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-017-3883-1"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12031101"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202103675"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108395"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155813"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0232-1"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3780"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.113310"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e41485"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-018-0249-1"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16301"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866530666221124122507"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8090334"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2024.03.028"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-022-01757-6"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-023-01705-3"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000515420"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-00894-w"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01841-1"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.30852"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.559384"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409764102"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12192-022-01297-7"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/0118761429323760240712050006"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pharmtox-032723-110507"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-022-00794-0"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2022.04.031"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-016-2253-7"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.97"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202503604RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202503604RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202503604RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T18:38:10Z","timestamp":1786559890000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202503604RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,18]]},"references-count":38,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,3,31]]}},"alternative-id":["10.1096\/fj.202503604RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202503604rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,18]]},"assertion":[{"value":"2025-10-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71629"}}