{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T21:41:42Z","timestamp":1782164502284,"version":"3.54.5"},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170789\uff1b32100563"],"award-info":[{"award-number":["32170789\uff1b32100563"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,2,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bladder cancer (BLCA) remains a highly prevalent and aggressive malignancy of the urinary tract, characterized by frequent recurrence and metastasis. Mediator complex subunit 15 (MED15) functions as a key transcriptional coactivator bridging transcription factors and RNA polymerase II, yet its role in BLCA progression has not been elucidated. Here, we identify MED15 as markedly upregulated in BLCA tissues. Gene set enrichment analysis (GSEA) revealed that MED15 expression correlates with epithelial\u2013mesenchymal transition (EMT)\u2010related pathways. Functionally, loss\u2010 and gain\u2010of\u2010function assays demonstrated that MED15 promotes EMT and enhances cell migration in a YAP1\u2010dependent manner. Mechanistically, MED15 interacts with YAP1 and stabilizes it by attenuating TRIM11\u2010mediated ubiquitination. Furthermore, under sorbitol\u2010induced stress conditions\u2014mimicking selected features of the bladder urothelium\u2014MED15 forms stress\u2010inducible, protein condensates and exhibits increased colocalization with YAP1. Collectively, these findings uncover a previously unrecognized MED15\u2010TRIM11\u2010YAP1 regulatory axis and support a model in which stress\u2010induced MED15 condensates are associated with YAP1 stabilization and pro\u2010metastatic behavior in bladder cancer.<\/jats:p>","DOI":"10.1096\/fj.202504160rr","type":"journal-article","created":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:11:02Z","timestamp":1770991862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Role of\n                    <scp>MED15<\/scp>\n                    in Enhancing\n                    <scp>EMT<\/scp>\n                    and Metastasis in Bladder Cancer Through\n                    <scp>YAP1<\/scp>\n                    Stabilization"],"prefix":"10.1096","volume":"40","author":[{"given":"Zi\u2010Xuan","family":"Liu","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010Yuan","family":"Shi","sequence":"additional","affiliation":[{"name":"GMU\u2010GIBH Joint School of Life Sciences, The Guangdong\u2010Hong Kong\u2010Macao Joint Laboratory for Cell Fate Regulation and Diseases Guangzhou Medical University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di\u2010Sheng","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Na","family":"Chen","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue\u2010Qi","family":"Liu","sequence":"additional","affiliation":[{"name":"Biomedical Imaging Core Facility of the Bio\u2010Tech Center Shenzhen Medical Academy of Research and Translation  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Ting","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Interventional Medicine Affiliated Hospital of Guizhou Medical University  Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Ting","family":"Liang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi\u2010Ni","family":"Huang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yan","sequence":"additional","affiliation":[{"name":"Experimental Research Department, the Fifth Affiliated Hospital Sun Yat\u2010Sen University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010kang","family":"Qiao","sequence":"additional","affiliation":[{"name":"Centre for Biological Science and Technology, Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, Department of Biology, Faculty of Arts and Sciences, Guangdong Zhuhai\u2010Macao Joint Biotech Laboratory Beijing Normal University  Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pancreatic Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin's Clinical Research Center for Cancer, Tianjin Key Laboratory of Digestive Cancer Key Laboratory of Cancer Prevention and Therapy  Tianjin Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"Cancer Research Center, School of Medicine Xiamen University  Xiamen Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Qi","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Tumor Immunology and Microenvironmental Regulation Guilin Medical University  Guilin Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatovenereology The Seventh Affiliated Hospital of Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sze\u2010Hoi","family":"Chan","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2759-5185","authenticated-orcid":false,"given":"Xing\u2010Ding","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"},{"name":"Key Laboratory for Efficacy and Safety Evaluation of Hematological Malignancy Targeted Medicine of Guangdong Provincial Drug Administration, School of Medicine, Shenzhen Campus of Sun Yat\u2010Sen University Sun Yat\u2010Sen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,13]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.22"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(22)01188\u20106"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568\u2010020\u201000313\u20101"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14767"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.01.028"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg0635"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915\u2010021\u201001178\u2010y"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010024\u201001944\u20101"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.13860"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2025.108296"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjs054"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.28647"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2020.12.018"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15194701"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41556\u2010020\u201000588\u20104"},{"issue":"6","key":"e_1_2_12_17_1","first-page":"3589","article-title":"VASN Promotes YAP\/TAZ and EMT Pathway in Thyroid Carcinogenesis in Vitro","volume":"11","author":"Bhandari A.","year":"2019","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.104092"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1843810"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964\u2010022\u201000898\u20100"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432\u2010013\u20101541\u2010y"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16034560"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.66"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201019\u20102388"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010022\u201005474\u20105"},{"key":"e_1_2_12_26_1","first-page":"283","volume-title":"Advances in Cancer Research","author":"Ko S.","year":"2022"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201642681"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.333435.119"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010022\u201001191\u20109"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.06.004"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e20183"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar3958"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.10.042"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010021\u201003572\u20106"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18478"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2021.110844"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010021\u201092054\u2010w"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222011014"},{"key":"e_1_2_12_39_1","unstructured":"Phase Separation of YAP Reorganizes Genome Topology for Long\u2010 Term YAP Target Gene Expression.Pdf."},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.70395"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.102023.1"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1109"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2015.1064854"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac246"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13242107"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010020\u201001161\u20101"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11693"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420\u2010024\u201001943\u20102"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010018\u20100316\u2010y"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201059309\u2010w"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010006\u20100328\u2010x"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010010\u20100505\u20109"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.10.048"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.11.002"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14079"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215367110"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E24\u201010\u20100436"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2014.1994"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202504160RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202504160RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202504160RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T14:42:35Z","timestamp":1773326555000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202504160RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,13]]},"references-count":57,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,2,28]]}},"alternative-id":["10.1096\/fj.202504160RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.202504160rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,13]]},"assertion":[{"value":"2025-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71558"}}