{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:51:05Z","timestamp":1781967065274,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The fat mass and obesity\u2010associated gene (\n                    <jats:italic>FTO<\/jats:italic>\n                    ), the first obesity\u2010related gene and m6A demethylase, has well\u2010defined roles in rodents but remains poorly understood in large mammals. Here, we combined siRNA\u2010mediated knockdown, transcriptome sequencing, and bioinformatics to explore\n                    <jats:italic>FTO<\/jats:italic>\n                    function in porcine adipogenesis and embryo development.\n                    <jats:italic>FTO<\/jats:italic>\n                    silencing inhibited cell proliferation, promoted apoptosis, and suppressed adipogenic differentiation of adipose\u2010derived mesenchymal stem cells (ADSCs) by downregulating key adipogenic genes (\n                    <jats:italic>PPAR\u03b3<\/jats:italic>\n                    ,\n                    <jats:italic>C\/EBP\u03b1<\/jats:italic>\n                    ,\n                    <jats:italic>FABP4<\/jats:italic>\n                    ,\n                    <jats:italic>SCD<\/jats:italic>\n                    ,\n                    <jats:italic>ACC\u03b1<\/jats:italic>\n                    ,\n                    <jats:italic>LPL<\/jats:italic>\n                    ). Transcriptome analysis of CRISPR\/Cas9\u2010generated\n                    <jats:italic>FTO<\/jats:italic>\n                    \u2010knockout fetal fibroblasts revealed altered pathways related to the cell cycle, senescence, apoptosis, and metabolism, including PI3K\u2010Akt, Wnt, MAPK, and Apelin signaling. Using these knockout cells for somatic cell nuclear transfer (SCNT), we found that while\n                    <jats:italic>FTO<\/jats:italic>\n                    loss did not affect blastocyst formation, all cloned piglets carrying the mutation died postnatally. These results demonstrate that\n                    <jats:italic>FTO<\/jats:italic>\n                    is essential for porcine adipogenesis and postnatal survival of cloned embryos. The findings provided a new insight into the roles and mechanisms of\n                    <jats:italic>FTO<\/jats:italic>\n                    in fat metabolism and development in large mammals.\n                  <\/jats:p>","DOI":"10.1096\/fj.202504686rr","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T12:40:04Z","timestamp":1780922404000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>FTO<\/scp>\n                    Regulates Porcine Adipogenesis and Postnatal Survival of Cloned Embryos"],"prefix":"10.1096","volume":"40","author":[{"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Germplasm Innovation, College of Animal Science and Technology Anhui Agricultural University  Hefei China"},{"name":"Center for Reproductive Medicine The Second Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengya","family":"Zhang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Germplasm Innovation, College of Animal Science and Technology Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Germplasm Innovation, College of Animal Science and Technology Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunlong","family":"Hu","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Germplasm Innovation, College of 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