{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:51:05Z","timestamp":1781967065298,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270856"],"award-info":[{"award-number":["82270856"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870547"],"award-info":[{"award-number":["81870547"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81670731"],"award-info":[{"award-number":["81670731"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010882","name":"Tianjin Municipal Education Commission","doi-asserted-by":"publisher","award":["2019ZD028"],"award-info":[{"award-number":["2019ZD028"]}],"id":[{"id":"10.13039\/501100010882","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Axin1, a scaffold protein, participates in maintaining glucose homeostasis and muscle function. However, the role of Axin1 in regulating skeletal muscle growth and regeneration remains poorly understood. This study aims to investigate the role of Axin1 in skeletal muscle proliferation, differentiation, and regeneration in the context of insulin resistance or obesity. We found that knockdown of Axin1 decreased the protein level of Cyclin D1 and increased the protein levels of MyoG and MyHC, but without effect on MyoD protein levels, to inhibit proliferation and promote differentiation of C2C12 muscle cells. Over\u2010expression of Axin1 had the opposite effect. Chronic insulin treatment up\u2010regulated Axin1 protein levels, which promoted myoblast proliferation and impaired differentiation in C2C12 muscle cells. Knockdown of Axin1 reversed these effects by inducing Cyclin D1\u2010mediated cell cycle arrest and up\u2010regulating MyoG expression. AAV\u2010siAxin1 significantly increased the protein and mRNA levels of MyoG of basal and CTX\u2010injured mouse skeletal muscle, without effect on Pax7 and MyoD. Skeletal muscle of HFD\u2010fed mice and\n                    <jats:italic>db\/db<\/jats:italic>\n                    mice have higher Axin1 and lower MyoG, eMyHC, and Desmin protein levels, which correlated with reduced early muscle regeneration in injured muscle. Knockdown of Axin1 reversed the effect of HFD on muscle regeneration. In summary, down\u2010regulation of Axin1 inhibits muscle cell proliferation and promotes differentiation in basal and insulin resistant conditions, and promotes skeletal muscle regeneration in HFD mice.\n                  <\/jats:p>","DOI":"10.1096\/fj.202505000r","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:31:21Z","timestamp":1780489881000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Axin1 Suppresses Muscle Regeneration by Promoting Myoblast Proliferation and Inhibiting Differentiation"],"prefix":"10.1096","volume":"40","author":[{"given":"Yingying","family":"Yue","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Tianjin First Central Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, General Hospital Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, NHC Key Lab of Hormones and Development, Tianjin Medical University Chu Hsien\u2010I Memorial Hospital &amp; Institute of Endocrinology Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, NHC Key Lab of Hormones and Development, Tianjin Medical University Chu Hsien\u2010I Memorial Hospital &amp; Institute of Endocrinology Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu","family":"Gu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, NHC Key Lab of Hormones and Development, Tianjin Medical University Chu Hsien\u2010I Memorial Hospital &amp; Institute of Endocrinology Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Tianjin First Central Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Tianjin First Central Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7481-1576","authenticated-orcid":false,"given":"Wenyan","family":"Niu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Tianjin Institute of Immunology, NHC Key Lab of Hormones and Development, Tianjin Medical University Chu Hsien\u2010I Memorial Hospital &amp; Institute of Endocrinology Tianjin Medical University  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1249\/JES.0000000000000227"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.665232"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384101.2015.1026519"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.12.020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.74220.1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101843"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-024-01482-4"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2013.09.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80324-4"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.pmbts.2017.11.026"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.10.006"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.169391"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.03.025"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202101094R"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/dom.16066"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-0647"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.02.014"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mito.2021.10.001"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01509-5"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00272.2019"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610185200"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5363.596"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08356"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2007.10.018"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004349200"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01682-07"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07638-4"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232113380"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600346"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-1155"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1100\/tsw.2011.137"},{"key":"e_1_2_10_33_1","first-page":"241","article-title":"Intestinal Epithelial Axin1 Deficiency Protects Against Colitis via Altered Gut Microbiota","volume":"35","author":"Garrett S.","year":"2024","journal-title":"Engineering (Beijing)"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56967-8"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2020.104667"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00462.2020"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/57.5.M326"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13252"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.9b01252"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202505000R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202505000R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202505000R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:08:12Z","timestamp":1781964492000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202505000R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,3]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6,15]]}},"alternative-id":["10.1096\/fj.202505000R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202505000r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,3]]},"assertion":[{"value":"2025-12-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e72016"}}