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Circular RNAs (circRNAs), a class of non\u2010coding RNAs with covalently closed loop structures, have recently garnered increasing attention in biological research. Growing evidence suggests that circRNAs are involved in the fine\u2010tuned regulation of TD in broiler through competitive endogenous RNA (ceRNA) networks. However, the role of circRNA in TD pathogenesis via protein\u2010binding mechanisms remains unclear. In our previous study, we identified circCOG6 (circ_0002951), a circular RNA derived from the COG6 gene, as significantly upregulated in a Thiram\u2010induced TD model, indicating its potential critical role in TD onset and progression. In\u00a0vitro functional assays revealed that overexpression of circCOG6 suppresses proliferation and differentiation of TD chondrocytes and promotes apoptosis. Mechanistically, through AGO2\u2010RIP, RNA pull\u2010down, and RIP experiments, we demonstrated that circCOG6 interacts with activin receptor type\u20101\u2010like (ACVRL1) to inhibit chondrocyte proliferation and differentiation, promote apoptosis, and synergistically enhance BMP\/Smad signaling activation, thereby contributing to TD pathogenesis. Furthermore, in\u00a0vivo studies showed that intra\u2010articular injection of adeno\u2010associated virus carrying shRNA targeting circCOG6 (AAV\u2010sh\u2010circCOG6) alleviates TD lesions in a broiler chicken. In conclusion, this study is the first to elucidate a circRNA\u2010mediated regulatory mechanism in broiler TD via RBP\u2010binding protein interaction, thereby enriching the TD regulatory network and offering a potential therapeutic target for its treatment.<\/jats:p>","DOI":"10.1096\/fj.202600203rr","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T12:37:27Z","timestamp":1779453447000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CircCOG6<\/scp>\n                    Suppresses Proliferation and Differentiation of Chicken Chondrocytes in Tibial Dyschondroplasia by Targeting ACVRL1 Protein"],"prefix":"10.1096","volume":"40","author":[{"given":"Xuyang","family":"Ji","sequence":"first","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P. R. 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