{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:50:56Z","timestamp":1781967056907,"version":"3.54.5"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T00:00:00Z","timestamp":1780099200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82370765"],"award-info":[{"award-number":["82370765"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82270802"],"award-info":[{"award-number":["82270802"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82570893"],"award-info":[{"award-number":["82570893"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82400895"],"award-info":[{"award-number":["82400895"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82500930"],"award-info":[{"award-number":["82500930"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Enhancer of zeste homolog 2 (EZH2), a well\u2010known methyltransferase, mediates histone H3 lysine 27 trimethylation (H3K27me3) and plays a critical role in various kidney diseases. Previous studies have demonstrated that EZH2 contributes to ischemia\u2013reperfusion (I\/R)\u2010induced acute kidney injury (AKI). However, the specific role and regulation of EZH2 in renal I\/R injury remain incompletely understood. This study provides a comprehensive analysis of EZH2's role and underlying mechanisms in renal ischemia\u2013reperfusion injury. Inhibition of EZH2 reduces apoptosis and ameliorates I\/R\u2010induced AKI in both in\u00a0vitro and in\u00a0vivo models. Global gene expression analysis by RNA\u2010seq and ChIP\u2010seq reveals a marked upregulation of Spalt\u2010Like Transcription Factor 1 (SALL1) and its involvement in the Wnt signaling pathway in EZH2\u2010knockdown HK\u20102 cells. Mechanistically, Cancer Susceptibility Candidate 15 (CASC15) recruits EZH2 to the SALL1 promoter, where EZH2 catalyzes H3K27me3 modification. ChIP analysis further confirms the enrichment of EZH2 at the SALL1 promoter. Additionally, silencing of SALL1 exacerbates hypoxia\u2010induced apoptosis in HK\u20102 cells through inactivation of \u03b2\u2010catenin. In conclusion, CASC15 recruits EZH2 to the SALL1 promoter, where EZH2 mediates H3K27me3 modification to suppress SALL1, thereby regulating the Wnt\/\u03b2\u2010catenin signaling pathway in renal I\/R injury. The CASC15\/EZH2\/SALL1 axis represents a promising therapeutic target for the treatment of AKI.<\/jats:p>","DOI":"10.1096\/fj.202600634r","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T16:29:41Z","timestamp":1780158581000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>LncRNA CASC15<\/scp>\n                    \u2010\n                    <scp>EZH2<\/scp>\n                    Interaction Promotes Ischemia\u2013Reperfusion Induced Acute Kidney Injury via Regulating\n                    <scp>SALL1<\/scp>\n                    and Wnt\/\u03b2\u2010Catenin Pathway"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3929-8033","authenticated-orcid":false,"given":"Mingjie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinzhou","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Urology Affiliated Suzhou Hospital of Nanjing Medical University  Suzhou Jiangsu China"},{"name":"Gusu School Nanjing Medical University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqin","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shanghai Changzheng Hospital, Naval Medical University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehua","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bojun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao","family":"Chi","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8095-8766","authenticated-orcid":false,"given":"Ming","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Nephrology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3471-6221","authenticated-orcid":false,"given":"Fan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"},{"name":"Taikang Center for Life and Medical Sciences of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Urology Renmin Hospital of Wuhan University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,30]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)32563-2"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20194941"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2017.184"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2507"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2012.tb00431.x"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.kint.2017.07.030"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI45161"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2020.03.016"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2021.106033"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014030262"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00438-5"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-018-0103-6"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1573405617666211213110222"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.07.015"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01393"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2022.05.027"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.111212"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.16765"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.640700"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154390"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.05.012"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00762-6"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2015.153"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-020-00343-w"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2020.01.004"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-1755.2005.00626.x"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13246292"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04793-1"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102262"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01323"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.83353"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-019-1123-9"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.12187"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2020.12032"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4036"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-020-00937-8"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/nep.13466"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.885527"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05492-3"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-021-00837-8"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2012.146"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.17447"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-019-01221-3"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-05782-4"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01688-x"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-021-03466-5"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.173"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.23448"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.04.156"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202600634R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202600634R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202600634R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T14:08:35Z","timestamp":1781964515000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202600634R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,30]]},"references-count":49,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,6,15]]}},"alternative-id":["10.1096\/fj.202600634R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202600634r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,30]]},"assertion":[{"value":"2026-02-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71989"}}