{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:34:22Z","timestamp":1793255662511,"version":"4.5.0"},"reference-count":20,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Bone morphogenetic protein (BMP)\u20103 (osteogenin) is an important regulatory cytokine during development and acts as a tumor suppressor in several cancers. BMPs are synthesized as proproteins that are cleaved by proprotein convertases to release biologically active ligands. The BMP\u20103 mature ligand is recorded as 16\u2009kDa consisting of carboxy\u2010terminal Q363\u2010R472 (110 amino acids). The BMP\u20103 ligand sequence was determined based on the expectation that BMP ligands are similar in size and has not been experimentally verified. Here we show the BMP\u20103 ligand is S290\u2010R472 (183 amino acids) consistent with the approximately 25\u2009kDa BMP\u20103 ligand secreted from human fibroblasts. This larger ligand size is consistent with proprotein convertase cleavage site prediction and supported by analyses using liquid chromatography tandem mass spectrometry. Data are available via ProteomeXchange with identifier PXD072827. These data indicate that the human BMP\u20103 mature ligand is S290\u2010R472 and that earlier studies using recombinant human BMP\u20103 ligand Q363\u2010R472 are flawed.<\/jats:p>","DOI":"10.1096\/fj.202600858","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:44:57Z","timestamp":1792410297000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Human Bone Morphogenetic Protein\u20103 Mature Ligand Is Larger Than Recorded Indicating Earlier Publications Are Flawed"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6156-9159","authenticated-orcid":false,"given":"David E.","family":"Phelan","sequence":"first","affiliation":[{"name":"Conway Institute of Biomolecular and Biomedical Research UCD Dublin  Dublin 4 Ireland"},{"name":"School of Medicine UCD Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7463-0676","authenticated-orcid":false,"given":"Caitriona","family":"Scaife","sequence":"additional","affiliation":[{"name":"Conway Institute of Biomolecular and Biomedical Research UCD Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2014-934X","authenticated-orcid":false,"given":"Emma R.","family":"Dorris","sequence":"additional","affiliation":[{"name":"Conway Institute of Biomolecular and Biomedical Research UCD Dublin  Dublin 4 Ireland"},{"name":"School of Medicine UCD Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4575-8857","authenticated-orcid":false,"given":"John","family":"Russell","sequence":"additional","affiliation":[{"name":"School of Medicine UCD Dublin  Dublin 4 Ireland"},{"name":"Department of Pediatric Otorhinolaryngology Children's Health Ireland (Crumlin)  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9891-481X","authenticated-orcid":false,"given":"Madeline","family":"Murphy","sequence":"additional","affiliation":[{"name":"Conway Institute of Biomolecular and Biomedical Research UCD Dublin  Dublin 4 Ireland"},{"name":"School of Medicine UCD Dublin  Dublin 4 Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.2741\/1355"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/83810"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.103422"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2024.150497"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20083"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1435-1"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae1010"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3201241"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae979"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0012-1606(03)00223-9"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21561"},{"issue":"1","key":"e_1_2_9_13_1","first-page":"S56","article-title":"BMP3: To Be or Not to Be a BMP","volume":"83","author":"Bahamonde M. 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