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This study examined the effects of \u03bcG on synaptic signaling and neuromuscular aging in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    <\/jats:styled-content>\n                    . The D01 cohort, consisting of L4 larvae to young adults raised in \u03bcG, exhibited a downregulation of genes linked to synaptic signaling, dopamine response, locomotion, cuticle development, and mitochondrial metabolism. This was accompanied by altered synapse dynamics, reduced motility, and shorter body length. In \u03bcG, aged worms showed a reduction in collagen gene expression, increased abnormalities in motor neuron morphology, changes in synaptic vesicle dynamics, and a collapse of mitochondrial morphology in body wall muscles, highlighting exacerbated aging\u2010like phenotypes. The gentle\u2010touch mechanoreceptor MEC\u20104 was identified as a key mediator of \u03bcG\u2010induced body length reduction and changes in extracellular matrix gene expression.\n                    <jats:italic>mec\u20104<\/jats:italic>\n                    mutants did not show \u03bcG\u2010associated body shortening. The expression of most mechanoreceptor genes, including stretch\u2010activated channels\n                    <jats:italic>unc\u2010105<\/jats:italic>\n                    and\n                    <jats:italic>del\u20101<\/jats:italic>\n                    , was downregulated under \u03bcG conditions. Notably, the expression of\n                    <jats:italic>tmc\u20101<\/jats:italic>\n                    and\n                    <jats:italic>degt\u20101<\/jats:italic>\n                    mechanoreceptor genes was downregulated independently of MEC\u20104. Restoration of physical stimulation using culture medium with small beads in space mitigated many \u03bcG\u2010induced neuromuscular defects and expression alterations including those in mechanoreceptor genes. These results highlight the role of mechanical stimuli in maintaining neuromuscular integrity during spaceflight and suggest that restoring tactile input could counter health risks from reduced tactile stimulation during long\u2010term space missions.\n                  <\/jats:p>","DOI":"10.1096\/fj.202600867rr","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T11:25:26Z","timestamp":1781609126000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced Mechanical Tactile Stimulation Under Space Microgravity Affects Synaptic Signaling and Contributes to Neuromuscular Aging in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6920-0594","authenticated-orcid":false,"given":"Atsushi","family":"Higashitani","sequence":"first","affiliation":[{"name":"Graduate School of Life Sciences Tohoku 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