{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:28:53Z","timestamp":1790807333303,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T00:00:00Z","timestamp":1780963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470858"],"award-info":[{"award-number":["32470858"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,6,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>ZMYND11 is the sole known reader of H3.3K36me3. It has been characterized as a transcriptional repressor that fine\u2010tunes RNA polymerase II (Pol II) elongation through recognition of gene body\u2010localized H3K36me3, based primarily on earlier ChIP\u2010seq data showing predominant ZMYND11 occupancy at gene bodies. However, subsequent data suggest that ZMYND11 may also localize to promoter regions. Here, using CUT&amp;Tag assay, we demonstrate that ZMYND11 robustly occupies promoter regions in mouse ESCs and MEFs, with its promoter enrichment positively correlating with gene expression levels and Pol II occupancy. We further identify formaldehyde crosslinking as a critical factor causing signal loss at transcription start sites in conventional ChIP\u2010seq. Mechanistically, ZMYND11 deficiency reduces the pausing index of Pol II, H3.3, and H3K36me3, indicating impaired transcription initiation. ZMYND11 knockout in mouse ESCs induces transcriptomic changes, impairs cell proliferation, and aberrantly activates 2\u2010cell\u2010specific transcriptional programs via ROS accumulation. Our findings reveal a previously unappreciated role for ZMYND11 as a transcriptional initiator that stabilizes Pol II at promoters, establishing its essential function in maintaining embryonic stem cell homeostasis and advancing our understanding of its context\u2010dependent transcriptional regulatory mechanisms.<\/jats:p>","DOI":"10.1096\/fj.202601335r","type":"journal-article","created":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T15:20:57Z","timestamp":1781018457000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>H3<\/scp>\n                    .\n                    <scp>3K36me3<\/scp>\n                    Reader\n                    <scp>ZMYND11<\/scp>\n                    Localizes to Gene Promoter and Regulates Transcription Initiation"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2242-2397","authenticated-orcid":false,"given":"Yu","family":"Tian","sequence":"first","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010fan","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010hua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Center for Reproduction and Genetics, Department of Obstetrics and Gynecology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9332-9408","authenticated-orcid":false,"given":"Li\u2010quan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Health, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4064-3970","authenticated-orcid":false,"given":"Ying","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Basic Medicine, Tongji Medical College Huazhong University of Science and Technology  Wuhan Hubei P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2019.10.007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2017.11"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2015.09.007"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2022.04.010"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-023-01145-3"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.08.061"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.10.039"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13045"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.347705"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400366"},{"issue":"1","key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"9010","DOI":"10.1038\/s41467-025-64063-0","article-title":"ZMYND11 Functions in Bimodal Regulation of Latent Genes and Brain\u2010Like Splicing to Safeguard Corticogenesis","volume":"16","author":"Chang X.","year":"2025","journal-title":"Nature Communications"},{"issue":"8","key":"e_1_2_10_13_1","first-page":"4452","article-title":"Interaction With ZMYND11 Mediates Opposing Roles of Ras\u2010Responsive Transcription Factors ETS1 and ETS2","volume":"45","author":"Plotnik J. P.","year":"2017","journal-title":"Nucleic Acids Research"},{"issue":"1","key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/s41467-020-20820-x","article-title":"Dual Functions of SPOP and ERG Dictate Androgen Therapy Responses in Prostate Cancer","volume":"12","author":"Bernasocchi T.","year":"2021","journal-title":"Nature Communications"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2533-0"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.08.022"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110947"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30940-1"},{"issue":"6","key":"e_1_2_10_19_1","doi-asserted-by":"crossref","DOI":"10.1093\/g3journal\/jkab101","article-title":"Effects of Sheared Chromatin Length on ChIP\u2010Seq Quality and Sensitivity","volume":"11","author":"Keller C. 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