{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T01:27:10Z","timestamp":1793582830096,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFD1800700"],"award-info":[{"award-number":["2025YFD1800700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018554","name":"Science and Technology Program of Gansu Province","doi-asserted-by":"publisher","award":["24JRRA018"],"award-info":[{"award-number":["24JRRA018"]}],"id":[{"id":"10.13039\/501100018554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>African swine fever (ASF) is an acute, febrile, and highly contagious infectious disease of swine with the etiological agent of African swine fever virus (ASFV). The mortality rate of virulent strains is as high as 100%. Strengthening biosafety is so far the most effective way to prevent and control ASF. Therefore, it is urgent to develop a safe and effective vaccine. In this study, a Genotype II live\u2010attenuated ASF vaccine bearing 24 genes deletion in 3 independent regions was constructed based on the highly virulent Eurasian strain ASFV CN\/GS 2018 backbone. The resulting mutant ASFV\u2010\u039424 is characterized by complete deletion of 24 genes distributed in 3 genomic positions of 852 to 11\u2009468, 19\u2009732 to 22\u2009929, and 179\u2009519 to 180\u2009617, among which MGF100 and whole MGF300 families are pioneeringly removed. The ASFV\u2010\u039424 displayed a delayed and reduced replication kinetics as well as aberrant icosahedral empty particles devoid of a nucleoid when compared to the parental virus. Animal experiments showed that ASFV\u2010\u039424 was completely attenuated in animals as evidenced by stable body temperature and no ASF\u2010compatible clinical signs in vaccinated pigs. The ASFV\u2010\u039424 could provide complete homologous protection against lethal challenge, as vaccinated pigs demonstrated boosted antibody response, transient but low levels of viremia in blood and virus titers in organs as well as almost undetectable viral shedding. Gene deletions in multiple regions are helpful for prevention of virulence reversion. These results indicate that ASFV\u2010\u039424 can be used as an effective and promising candidate vaccine to control the spread of ASFV.<\/jats:p>","DOI":"10.1096\/fj.202601491r","type":"journal-article","created":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T15:46:50Z","timestamp":1788364010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genotype\n                    <scp>II<\/scp>\n                    Live\u2010Attenuated\n                    <scp>ASFV<\/scp>\n                    Vaccine Bearing 24 Genes Deletion in 3 Independent Regions Is Able to Provide Complete Protection Against Homologous Lethal Challenge"],"prefix":"10.1096","volume":"40","author":[{"given":"Fan","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Dang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Du","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwang","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Tian","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jijun","family":"He","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6850-1379","authenticated-orcid":false,"given":"Haixue","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.578474"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-62736-y"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1804.111813"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00215"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105602"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2411.181274"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000991"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001049"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001637"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/jvetres-2017-0017"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13122552"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11020199"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2016.11.021"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12431"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15102064"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14092024"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01760-16"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00554-15"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines12101114"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2023.2300464"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00329-22"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02330-21"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2018.04.002"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13081473"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00969-15"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01058-17"},{"key":"e_1_2_12_28_1","first-page":"104","article-title":"Hemadsorption and Cytopathic Effect Produced by African Swine Fever Virus in Swine Bone Marrow and Buffy Coat Cultures","volume":"21","author":"Malmquist W. A.","year":"1960","journal-title":"American Journal of Veterinary Research"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2022.109594"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00789-20"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.06.008"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13243807"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-021-00350-6"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01667-21"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/v16060949"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02677-24"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202201856R"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011580"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/v17030309"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.818969"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1382675"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12250-020-00304-4"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00690-25"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2210808120"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11427-020-1657-9"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2026.2640697"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-026-01377-0"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41541-026-01399-8"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3123"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2001110"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2016.03.019"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202601491R","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.202601491R","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.202601491R","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T00:50:50Z","timestamp":1793580650000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.202601491R"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,2]]},"references-count":50,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2026,9,15]]}},"alternative-id":["10.1096\/fj.202601491R"],"URL":"https:\/\/doi.org\/10.1096\/fj.202601491r","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,2]]},"assertion":[{"value":"2026-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e72218"}}