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Whether this fragmentation promotes or prevents cell death and whether the mitochondrial dynamics machinery plays a role are unresolved. To address these questions, we measured the effect of hypoxia on mitochondrial morphology in a\n                    <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                    Raptor mutant resistant to hypoxic death and in mutants with disrupted mitochondrial fission and fusion. The Raptor loss\u2010of\u2010function mutant reduced hypoxia\u2010induced mitochondrial fragmentation and death. However, forcing mitochondrial fragmentation prior to hypoxia by combining the Raptor mutation with a loss\u2010of\u2010function mutation in mitofusin did not increase hypoxic death. A loss\u2010of\u2010function mutation in\n                    <jats:italic>drp\u20101<\/jats:italic>\n                    , which is required for mitochondrial fission, did not block hypoxia\u2010induced mitochondrial fragmentation nor enhance Raptor hypoxia resistance; rather,\n                    <jats:italic>drp\u20101<\/jats:italic>\n                    (lf) was surprisingly mildly hypoxia resistant and partially suppressed the high\u2010level hypoxia resistance of the Raptor mutant. Likewise, loss of DRP\u20101 function interacted synthetically with the Raptor(lf) mutant to produce tangled mitochondria, demonstrating a role of Raptor in maintenance of the mitochondrial network. Vitamin B12 supplementation and feeding with a bacterial strain replete in vitamin B12 mitigated hypoxia\u2010induced mitochondrial fragmentation. Our results demonstrate that fragmented mitochondria do not necessarily promote hypoxic cell death, and hypoxia\u2010induced mitochondrial fragmentation is mechanistically distinct from physiological mitochondrial fission.\n                  <\/jats:p>","DOI":"10.1096\/fj.202601561r","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T07:58:31Z","timestamp":1782719911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Raptor and Drp1 Function Synthetically to Control Hypoxic Death and the Mitochondrial Network in\n                    <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1096","volume":"40","author":[{"given":"Julien","family":"Goldstick","sequence":"first","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine University of Washington School of Medicine  Seattle Washington USA"},{"name":"Mitochondria and Metabolism Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"Compte","sequence":"additional","affiliation":[{"name":"Drexel University College of Medicine  Philadelphia Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Ling","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine University of Washington School of Medicine  Seattle Washington USA"},{"name":"Mitochondria and Metabolism Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison E.","family":"Ritter","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology and Pain Medicine University of Washington School of Medicine  Seattle Washington USA"},{"name":"Mitochondria and Metabolism Center University of Washington  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2562-4392","authenticated-orcid":false,"given":"C. 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