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Post\u2010transcriptional regulation exerts important functions in Sertoli cells. Complex alternative splicing processes exist in Sertoli cells. This study focuses on splicing factor SRSF1 to investigate its function and mechanisms in Sertoli cell development. Sertoli cell\u2010specific SRSF1 ablation from embryonic development arrests cell proliferation and increases apoptosis, thereby reducing Sertoli cell number, constricting the germ cell microenvironment, and causing progressive germ cell loss, ultimately resulting in male infertility. Mechanistically, SRSF1 regulates CDK pathway and Bax\/Bcl2 pathway\u2010related gene transcription and forms spliceosomal complexes with DDX5 and RBMX\/RBMXL1 to modulate AS of\n                    <jats:italic>Cidea<\/jats:italic>\n                    and\n                    <jats:italic>Rif1<\/jats:italic>\n                    , thereby governing Sertoli cell proliferation, apoptosis, and DNA damage response. This study establishes SRSF1 as a multifunctional spliceosomal hub in Sertoli cells, refining our understanding of post\u2010transcriptional regulation in spermatogenesis and providing potential therapeutic targets for male infertility.\n                  <\/jats:p>","DOI":"10.1096\/fj.202601693r","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:15:11Z","timestamp":1789974911000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>SRSF1<\/scp>\n                    Maintains Sertoli Cell Proliferation and Survival by Regulating Alternative Splicing in Coordination With\n                    <scp>DDX5<\/scp>\n                    and\n                    <scp>RBMX<\/scp>\n                    \/\n                    <scp>RBMXL1<\/scp>"],"prefix":"10.1096","volume":"40","author":[{"given":"Shuang","family":"Tian","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Lv","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"},{"name":"Department of Physiology Harbin Medical University  Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longjie","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaguang","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaofan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Congxin","family":"Chai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexue","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7625-0757","authenticated-orcid":false,"given":"Xiaomei","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohong","family":"Yao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6214-569X","authenticated-orcid":false,"given":"Jiali","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, College of Biological Sciences China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2025.1506229"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12978-025-01966-7"},{"issue":"4","key":"e_1_2_15_4_1","first-page":"e184","article-title":"Fertility and the Aging Male","volume":"13","author":"Harris I. 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