{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T09:23:57Z","timestamp":1787822637088,"version":"build-2784847793"},"reference-count":41,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,8,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The ovary orchestrates female reproduction, where cellular composition and intercellular communication dictate reproductive cycles. Despite advances in chelonian reproductive biology, the cellular composition and regulatory mechanisms of the ovary remain unclear, thereby restricting insights into reproductive ecology and conservation strategies. Here, we performed single\u2010cell RNA sequencing (scRNA\u2010seq) to systematically characterize the molecular signatures of ovarian cell types in the Chinese pond turtle (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mauremys reevesii<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). By integrating differential gene expression analysis and functional enrichment, we constructed a high\u2010resolution ovarian cell atlas and identified major cell populations, including granulosa cells and oocytes. Specifically, we identified\n                    <jats:italic>Wnt family member 4<\/jats:italic>\n                    (\n                    <jats:italic>WNT4<\/jats:italic>\n                    ) and\n                    <jats:italic>Wnt family member 6<\/jats:italic>\n                    (\n                    <jats:italic>WNT6<\/jats:italic>\n                    ) as specific markers for granulosa cells and\n                    <jats:italic>Ubiquitin C\u2010terminal hydrolase L1<\/jats:italic>\n                    (\n                    <jats:italic>UCHL1<\/jats:italic>\n                    ) for oocytes. Furthermore, the specific expression of these granulosa cell markers was validated using dual\u2010color in\u00a0situ hybridization. Collectively, this study elucidates the ovarian biology of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. reevesii<\/jats:italic>\n                    <\/jats:styled-content>\n                    at the cellular and molecular levels, providing a vital theoretical foundation for understanding chelonian reproductive ecology and offering important insights into reproductive ecology in turtles.\n                  <\/jats:p>","DOI":"10.1096\/fj.202601922rr","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:10:10Z","timestamp":1787681410000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of Ovarian Cell Types and Molecular Signatures in\n                    <i>Mauremys reevesii<\/i>\n                    Using Single\u2010Cell Sequencing"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1963-1534","authenticated-orcid":false,"given":"Kaiqi","family":"Yuan","sequence":"first","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0373-6140","authenticated-orcid":false,"given":"Xin","family":"Niu","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9225-0560","authenticated-orcid":false,"given":"Yue","family":"Yuan","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2787-2193","authenticated-orcid":false,"given":"Jiale","family":"Zhou","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8567-4415","authenticated-orcid":false,"given":"Meiling","family":"Hong","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1340-5333","authenticated-orcid":false,"given":"Li","family":"Ding","sequence":"additional","affiliation":[{"name":"Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences Hainan Normal University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1434.009"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.11.002"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.08.031"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP\u201020\u20100471"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/biolre\/ioab065"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.15848"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/7064179"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2014.08.002"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2021.07.083"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.01.009"},{"key":"e_1_2_11_12_1","first-page":"106","article-title":"Review 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