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Although the\n                    <jats:italic>TFEB<\/jats:italic>\n                    gene is highly expressed in the testes, the mechanism by which it affects male fertility remains unclear. Here, we report that spermatogonia\u2010specific deletion of TFEB in mice results in a multifaceted reproductive phenotype, including impaired fertility, compromised sperm motility, and attenuated androgen production. Immunofluorescence results showed a significant decrease in the expression levels of TNP1, a marker for spermiogenesis. Under electron microscopy, we observed abnormalities in the mitochondria of the testes and sperm. Integrated transcriptomic and biochemical analyses identified a cluster of mitochondrial\u2010associated genes, including Star, Slc25a48, Gss, and ROMO1, with functional enrichment pinpointing disruptions in steroidogenic flux and calcium homeostasis. In summary, our study identifies\n                    <jats:italic>TFEB<\/jats:italic>\n                    as a pivotal regulator of mitochondrial integrity in the testes, the loss of which drives male subfertility through metabolic and hormonal dysregulation.\n                  <\/jats:p>","DOI":"10.1096\/fj.202601937rr","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T19:52:06Z","timestamp":1785959526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>TFEB<\/scp>\n                    Deficiency Impairs Male Fertility Through Mitochondrial Dysfunction"],"prefix":"10.1096","volume":"40","author":[{"given":"Hongxia","family":"Li","sequence":"first","affiliation":[{"name":"Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital  Taiyuan China"},{"name":"Third Hospital of Shanxi Medical University, 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Hospital, Shanxi Academy of Medical Sciences Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le","family":"Zhao","sequence":"additional","affiliation":[{"name":"Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanqi","family":"Yu","sequence":"additional","affiliation":[{"name":"Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital  Taiyuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital  Taiyuan 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