{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:06:12Z","timestamp":1789052772739,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["25JRRA437"],"award-info":[{"award-number":["25JRRA437"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    African swine fever virus (ASFV) causes an incurable swine disease with nearly 100% mortality, posing a catastrophic threat to global pig production. The soft tick\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ornithodoros lahorensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    acts as a critical biological vector that sustains persistent ASFV replication and mediates long\u2010distance viral transmission, yet the molecular mechanisms governing ASFV\u2010tick interplay remain poorly understood. Here, we integrated transcriptomics and metabolomics to systematically dissect molecular changes in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>O.\u00a0lahorensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    across three infection stages: Uninfected control, early infection (7\u2009days post\u2010infection, dpi), and late persistent infection (21 dpi). Multi\u2010omics integration revealed that ASFV extensively remodels tick host metabolism, predominantly activating purine\/pyrimidine metabolism, lipid biosynthesis, and energy metabolism. We further characterized a conserved regulatory module consisting of 12 core genes and 8 signature metabolites that collectively support ASFV genome replication and virion assembly. Three hub metabolic genes (\n                    <jats:italic>TK1<\/jats:italic>\n                    ,\n                    <jats:italic>ATP5F1B<\/jats:italic>\n                    , and\n                    <jats:italic>IMPDH<\/jats:italic>\n                    ) were selected for functional validation via siRNA silencing in ticks; individual gene silencing suppressed ASFV loads by 89.2%, 91.5%, and 87.8%, respectively (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001***). This work represents the first comprehensive multi\u2010omics investigation of ASFV infection in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>O. lahorensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . We identified tick\u2010specific molecular targets to block vector\u2010mediated ASFV spread and established a standardized multi\u2010omics analytical pipeline for tick\u2013virus interaction research. Our findings elucidate the mechanistic basis of long\u2010term ASFV persistence in soft ticks and deliver novel actionable clues for developing vector\u2010targeted ASF intervention strategies.\n                  <\/jats:p>","DOI":"10.1096\/fj.202602001rr","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T12:47:50Z","timestamp":1788180470000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptomic and Metabolomic Profiling Identifies a Core Gene\u2013Metabolite Axis Driving African Swine Fever Virus Replication in the Soft Tick\n                    <scp>\n                      <i>Ornithodoros lahorensis<\/i>\n                    <\/scp>"],"prefix":"10.1096","volume":"40","author":[{"given":"Jin","family":"Luo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaifei","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Agriculture and Biological Sciences Yuxi Normal University  Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangyu","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"},{"name":"College of Veterinary Medicine Hunan Agricultural University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoqi","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"},{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Nanning Animal Disease Prevention and Control Center  Nanning China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoyun","family":"Ren","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4214-1212","authenticated-orcid":false,"given":"Shuaiyang","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Kashif","family":"Obaid","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiquan","family":"Guan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Lanzhou China"},{"name":"Jiangsu co\u2010Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis Yangzhou University  Yangzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2024.110277"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00215"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2024.1413237"},{"key":"e_1_2_11_5_1","first-page":"357","article-title":"The Habitat 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