{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T01:27:13Z","timestamp":1793582833113,"version":"4.5.0"},"reference-count":47,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"publisher","award":["CARS\u201037"],"award-info":[{"award-number":["CARS\u201037"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The pituitary gland is a key regulator of mammalian reproduction. By secreting the gonadotropins\u2014follicle\u2010stimulating hormone (FSH) and luteinizing hormone (LH)\u2014the anterior pituitary precisely coordinates reproductive function. The hypothalamus releases gonadotropin\u2010releasing hormone (GnRH), which in turn regulates the synthesis and secretion of gonadotropins by the anterior pituitary gland. Although GnRH\u2010dependent regulation of pituitary gonadotropins has been extensively studied in rodents, the mechanisms by which GnRH regulates gonadotropins in the bovine anterior pituitary gland remain unclear. In this research, we primarily used primary bovine anterior pituitary cells (PCs) for the experiments, and employed the mouse gonadotropin cell line L\u03b2T2 to further validate the regulatory effect of GnRH on pituitary gonadotropin\u2010secreting cells. We first demonstrated that GnRH promotes bovine gonadotropin synthesis and secretion and concomitantly reduces global cellular m7G methylation. Further experiments demonstrated that METTL1, a key methyltransferase responsible for m7G modification, is downregulated after GnRH treatment and functions as a negative regulator of gonadotropin synthesis and secretion. Mechanistically, experiments such as MeRIP\u2010qPCR and RNA stability analyses showed that METTL1 mediates m7G methylation of bovine FOXO1 mRNA, thereby modulating FOXO1 stability and protein expression, and regulating gonadotropin synthesis and secretion. Collectively, these findings demonstrate that GnRH regulates gonadotropin synthesis and secretion in the bovine anterior pituitary, and reveal an important role for RNA epigenetic modification in this process.<\/jats:p>","DOI":"10.1096\/fj.202602504rr","type":"journal-article","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:26:11Z","timestamp":1788881171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>GnRH<\/scp>\n                    Promotes Gonadotropin Synthesis and Secretion in the Bovine Anterior Pituitary by Inhibiting\n                    <scp>METTL1<\/scp>\n                    \u2010Mediated\n                    <scp>m7G<\/scp>\n                    Modification of\n                    <scp>FOXO1 mRNA<\/scp>"],"prefix":"10.1096","volume":"40","author":[{"given":"Yu","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing\u2010Bing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Xiang","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Xin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningbo Sansheng Biological Technology Co., Ltd.  Ningbo Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Animals, College of Animal Sciences Jilin University  Changchun Jilin 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