{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T06:34:16Z","timestamp":1793255656658,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2026,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Poultry skeletal muscle fiber composition, primarily defined by oxidative slow\u2010twitch and glycolytic fast\u2010twitch fibers, is a major determinant of meat quality. Yet the miRNA\u2010mRNA regulatory mechanisms controlling fiber type specification remain largely unresolved. Here, we compared the transcriptomic profiles of pectoralis major (PM) and soleus muscle (SM) in Tianfu broilers and identified gga\u2010miR\u20106631\u20105p as a key slow\u2010twitch\u2010associated microRNA (miRNA) through small RNA sequencing and weighted gene co\u2010expression network analysis. Mechanistically, acylphosphatase 2 (\n                    <jats:italic>ACYP2<\/jats:italic>\n                    ) was validated as a direct target of gga\u2010miR\u20106631\u20105p by bioinformatics prediction and dual\u2010luciferase reporter assays, and its expression was inversely correlated with miR\u20106631\u20105p. Functional analyses in primary chicken skeletal muscle satellite cells (SMSCs) revealed that miR\u20106631\u20105p significantly promotes cell proliferation and slow\u2010twitch fiber specification but suppresses myogenic differentiation. In contrast,\n                    <jats:italic>ACYP2<\/jats:italic>\n                    exerts opposite effects by inhibiting SMSCs' proliferation, enhancing myotube fusion, and repressing slow\u2010twitch fiber formation. Finally, rescue assays involving miR\u20106631\u20105p and\n                    <jats:italic>ACYP2<\/jats:italic>\n                    further validated the effect of this regulatory axis on muscle fiber type switching. Together, these results establish that the miR\u20106631\u20105p\/\n                    <jats:italic>ACYP2<\/jats:italic>\n                    axis is an important regulator of myogenesis and muscle fiber type specification, highlighting its potential for molecular improvement of poultry meat quality.\n                  <\/jats:p>","DOI":"10.1096\/fj.202602701rr","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:05:11Z","timestamp":1792508711000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>miR<\/scp>\n                    \u20106631\u20105p\/\n                    <scp>\n                      <i>ACYP2<\/i>\n                    <\/scp>\n                    Axis Orchestrates Myogenesis and Slow\u2010Twitch Muscle Fiber Specification in Broilers"],"prefix":"10.1096","volume":"40","author":[{"given":"Longge","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengyong","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiyu","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3477-1458","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"},{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University  Chengdu China"},{"name":"Key Laboratory of Livestock and Poultry Multi\u2010Omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology Sichuan Agricultural University  Chengdu P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods11040528"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2024.10.030"},{"key":"e_1_2_12_4_1","first-page":"873","article-title":"Poultry Meat Quality in Relation to Muscle Growth and Muscle Fiber Characteristics","volume":"37","author":"Ismail I.","year":"2017","journal-title":"Korean Journal for Food Science of Animal Resources"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2025.110009"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0321839"},{"key":"e_1_2_12_7_1","first-page":"4072","article-title":"Integrated miRNA\u2010mRNA Analysis Reveals Regulatory Mechanisms of Muscle Fiber Transformation Underlying Meat Quality Variation in Chicken","volume":"74","author":"Zhang D.","year":"2026","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14152225"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186732"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-025-00663-2"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200911036"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1725"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-022-04168-7"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.1008649"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.psj.2021.101537"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2026.148544"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2021.126172"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-60866-3"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b02193"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.137990"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1107s32"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-10-87"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-021-00664-1"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.141137"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21093274"},{"key":"e_1_2_12_27_1","first-page":"227","article-title":"Muscle Fiber Types","volume":"63","author":"Herbison G. 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