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As a nonfermenting bacterium, it relies on oxidative phosphorylation, in which the F\u2010ATP synthase, composed of an F\n                    <jats:sub>1<\/jats:sub>\n                    \u2010ATPase (\n                    <jats:italic>\u03b1<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    :\n                    <jats:italic>\u03b2<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    :\n                    <jats:italic>\u03b3<\/jats:italic>\n                    :\n                    <jats:italic>\u03b5<\/jats:italic>\n                    ) and F\n                    <jats:sub>O<\/jats:sub>\n                    \u2010domain (\n                    <jats:italic>a<\/jats:italic>\n                    :\n                    <jats:italic>b<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>2<\/jats:italic>\n                    <\/jats:sub>\n                    :\n                    <jats:italic>c<\/jats:italic>\n                    <jats:sub>10<\/jats:sub>\n                    ), synthesizes ATP, the cell's currency of life. Here, we present cryo\u2010electron microscopy structures of the F\n                    <jats:sub>1<\/jats:sub>\n                    \u2010ATPase captured during ATP synthesis, revealing concerted conformational changes within the\n                    <jats:italic>\u03b1<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    :\n                    <jats:italic>\u03b2<\/jats:italic>\n                    <jats:sub>3<\/jats:sub>\n                    \u2010catalytic head that trigger structural transitions of subunit\n                    <jats:italic>\u03b5<\/jats:italic>\n                    and twisting of the\n                    <jats:italic>\u03b3<\/jats:italic>\n                    :\n                    <jats:italic>\u03b5<\/jats:italic>\n                    central stalk, coupled with spontaneous ATP formation. Molecular dynamics simulations validate and expand the ensemble of structural rearrangements underlying the rotary mechanism. The resolved intermediate states of the\n                    <jats:italic>\u03b5<\/jats:italic>\n                    subunit reveal its role as a molecular gear, regulating ATP synthesis while simultaneously inhibiting ATP hydrolysis, essential for the pathogen to prevent wastage of ATP. These structural snapshots identify novel therapeutic targets and pave the way for rational design of selective inhibitors.\n                  <\/jats:p>","DOI":"10.1096\/fj.202602735r","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T13:25:48Z","timestamp":1792243548000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural Snapshots of\n                    <scp>\n                      F\n                      <sub>1<\/sub>\n                    <\/scp>\n                    \u2010\n                    <scp>ATPase<\/scp>\n                    Catalysis in Drug Resistant\n                    <scp>\n                      <i>Acinetobacter baumannii<\/i>\n                    <\/scp>\n                    Reveal Novel Therapeutic Targets"],"prefix":"10.1096","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2454-9463","authenticated-orcid":false,"given":"Khoa C. M.","family":"Le","sequence":"first","affiliation":[{"name":"School of Biological Sciences Nanyang Technological University  Singapore Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5493-8753","authenticated-orcid":false,"given":"Jan K.","family":"Marzinek","sequence":"additional","affiliation":[{"name":"Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)  Singapore Republic of Singapore"},{"name":"Program in Emerging Infectious Diseases Duke\u2010National University of Singapore Medical School  Singapore Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2934-278X","authenticated-orcid":false,"given":"Vikneswaran","family":"Mathiyazakan","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Nanyang Technological University  Singapore Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2900-098X","authenticated-orcid":false,"given":"Peter J.","family":"Bond","sequence":"additional","affiliation":[{"name":"Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)  Singapore Republic of Singapore"},{"name":"Department of Biological Sciences National University of Singapore  Singapore Republic of Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5730-8319","authenticated-orcid":false,"given":"Gerhard","family":"Gr\u00fcber","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Nanyang Technological University  Singapore Republic of Singapore"},{"name":"Bioinformatics Institute (BII), Agency for Science, Technology and Research (A*STAR)  Singapore Republic of 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