{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T02:08:17Z","timestamp":1773886097980,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[1997,11,1]],"date-time":"1997-11-01T00:00:00Z","timestamp":878342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1997,11]]},"abstract":"<jats:p>\n                    The ubiquitin\u2010proteasome pathway is the principal mechanism for the turnover of shortlived proteins in eukaryotic cells. In this pathway, the covalent ligation of ubiquitin to the substrate is a signal for recognition by the 26S proteasome. Recent studies indicate that targeting of substrates of the ubiquitin pathway to the proteasome is usually accomplished by the ligation of a polyubiquitin chain assembled through K48\u2010G76 isopeptide bonds, rather than by ligation of monoubiquitin. In addition to providing benefits in signal generation, recognition, and persistence, assigning the proteolytic targeting function to a specific specific type of polyubiquitin chain may allow monoubiquitin or polyubiquitin chains of novel structures to serve distinct targeting functions. Besides polyubiquitinated substrates, the proteasome also degrades an unknown number of proteins that are recognized without undergoing ubiquitination. Ornithine decarboxylase is the prototype ubiquitin\u2010independent substrate; it is targeted to the proteasome through noncovalent interaction with a specific protein factor known as antizyme. The existence of ubiquitin\u2010independent substrates of the proteasome raises important questions about the nature of the substrate\u2010 and protea\u2010some\u2010based elements that cooperate to bring about the targeting of substrates to this novel proteolytic complex.\u2014Pickart, C. M. Targeting of substrates to the 26S proteasome.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    11, 1055\u20131066 (1997)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.11.13.9367341","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T06:27:59Z","timestamp":1516256879000},"page":"1055-1066","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":293,"title":["Targeting of substrates to the 26S proteasome"],"prefix":"10.1096","volume":"11","author":[{"given":"Cecile M.","family":"Pickart","sequence":"first","affiliation":[{"name":"Department of Biochemistry School of Hygiene and Public Health Johns Hopkins University Baltimore Maryland 21205 USA"}]}],"member":"311","published-online":{"date-parts":[[1997,11]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.11.13.9367341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.11.13.9367341","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T16:34:35Z","timestamp":1761669275000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.11.13.9367341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,11]]},"references-count":0,"journal-issue":{"issue":"13","published-print":{"date-parts":[[1997,11]]}},"alternative-id":["10.1096\/fasebj.11.13.9367341"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.11.13.9367341","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,11]]},"assertion":[{"value":"1997-11-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}