{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T07:18:35Z","timestamp":1770707915416,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[1991,4,1]],"date-time":"1991-04-01T00:00:00Z","timestamp":670464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:p>\n                    Hepatocytes are stimulated to produce nitric oxide (NO\u00b7) from L\u2010arginine in response to conditioned Kupffer cell medium or a combination of cytokines. Associated with the production of NO\u00b7 in hepatocytes, there is a profound decrease in total protein synthesis ([\n                    <jats:sup>3<\/jats:sup>\n                    H]leucine incorporation). This report demonstrates that authentic NO\u00b7 and the NO\u00b7\u2010generating compound S\u2010nitroso\u2010N\u2010acetylpenicillamine inhibit hepatocyte total protein synthesis in a reversible and concentration\u2010dependent fashion. In parallel with the suppression of hepatocyte total protein synthesis, authentic NO\u00b7 inhibits the production of two specific hepatocyte proteins, albumin and fibrinogen, without influencing the quantity of albumin mRNA. Although authentic NO\u00b7 induces a rapid increase in cGMP levels in hepatocytes, the addition of the cGMP analog 8\u2010bromoguanosine 3\u2018:5\u2018 cyclic monophosphate to unstimulated HC cultures does not reproduce the inhibition of total protein synthesis. These data show that NO\u00b7 is the hepatocyte L\u2010arginine metabolite that inhibits protein synthesis. Furthermore, these findings indicate that NO\u00b7 does not inhibit hepatocyte protein synthesis solely through the activation of soluble guanylate cyclase but appears to affect a translational or posttranslational process.\u2014Curran, R. D.; Ferrari, F. K.; Kispert, P. H.; Stadler, J.; Stuehr, D. J.; Simmons, R. L.; Billiar, T. R. Nitric oxide and nitric oxide\u2010generating compounds inhibit hepatocyte protein synthesis.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    5: 2085\u20132092; 1991.\n                  <\/jats:p>","DOI":"10.1096\/fasebj.5.7.1707021","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T09:15:39Z","timestamp":1516266939000},"page":"2085-2092","source":"Crossref","is-referenced-by-count":171,"title":["Nitric oxide and nitric oxide\u2010generating compounds inhibit hepatocyte protein synthesis"],"prefix":"10.1096","volume":"5","author":[{"given":"Ronald D.","family":"Curran","sequence":"first","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]},{"given":"Forrest K.","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]},{"given":"Paul H.","family":"Kispert","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]},{"given":"Josef","family":"Stadler","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]},{"given":"Dennis J.","family":"Stuehr","sequence":"additional","affiliation":[{"name":"Department of Medicine Cornell University Medical College New York New York 10021 USA"}]},{"given":"Richard L.","family":"Simmons","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]},{"given":"Timothy R.","family":"Billiar","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Pittsburgh School of Medicine Pittsburgh Pennsylvania 15261 USA"}]}],"member":"311","published-online":{"date-parts":[[1991,4]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.5.7.1707021","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.5.7.1707021","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T20:09:05Z","timestamp":1761682145000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.5.7.1707021"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,4]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1991,4]]}},"alternative-id":["10.1096\/fasebj.5.7.1707021"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.5.7.1707021","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,4]]}}}