{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T19:31:43Z","timestamp":1761766303029,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[1995,9,1]],"date-time":"1995-09-01T00:00:00Z","timestamp":809913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:p>\n                    The most frequently found myositis\u2010specific antibody, the anti\u2010Jo\u20101 antibody (anti\u2010HRS), binds to histidyl\u2010tRNA synthetase (HRS). Although this antibody reacts with HRS, it is unclear whether HRS is the stimulating antigen or is merely a protein that cross\u2010reacts with a yet undefined antigen. Because antibody directed against an unrelated antigen would not be expected to cross\u2010react with HRS at multiple sites, we mapped the epitopes on HRS to resolve this issue. We found by Western blot analyses that immunoglobulins G (IgG) from 18 of 19 anti\u2010HRS positive patient sera react with amino acids 2\u201044 and 286\u2010509 of HRS. Patient IgG specific for these two epitopes were found not to inhibit HRS enzyme activity. Instead, the inhibitory property of anti\u2010HRS was found to be associated with antibodies that do not react to HRS in immunoblots, indicating the presence of other epitopes. In addition, antibodies that react in immunoblots were found to represent only a small fraction of total anti\u2010HRS antibody. Our finding that patient IgG recognized at least three distinct epitopes on HRS strongly suggests that the immunological response at some point in the disease is directed against HRS and not against a cross\u2010reactive anti\u2010gen.\u2014Martin, A., Shulman, M. J., Tsui, F. W. L. Epitope studies indicate that histidyl\u2010tRNA synthetase is a stimulating antigen in idiopathic myositis.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    9, 1226\u20101233 (1995)\n                  <\/jats:p>","DOI":"10.1096\/fasebj.9.12.7672516","type":"journal-article","created":{"date-parts":[[2018,1,18]],"date-time":"2018-01-18T05:33:06Z","timestamp":1516253586000},"page":"1226-1233","source":"Crossref","is-referenced-by-count":35,"title":["Epitope studies indicate that histidyl\u2010tRNA synthetase is a stimulating antigen in idiopathic myositis"],"prefix":"10.1096","volume":"9","author":[{"given":"Alberto","family":"Martin","sequence":"first","affiliation":[{"name":"The Arthritis Center\u2010Research Unit The Toronto Hospital Research Institute The Toronto Hospital Western Division Toronto Ontario M5T 2S8 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc J.","family":"Shulman","sequence":"additional","affiliation":[{"name":"Department of Immunology University of Toronto Toronto Ontario M5S 1A8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence W. L.","family":"Tsui","sequence":"additional","affiliation":[{"name":"The Arthritis Center\u2010Research Unit The Toronto Hospital Research Institute The Toronto Hospital Western Division Toronto Ontario M5T 2S8 Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,9]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.9.12.7672516","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fasebj.9.12.7672516","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T23:51:24Z","timestamp":1761695484000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.9.12.7672516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1096\/fasebj.9.12.7672516"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.9.12.7672516","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"type":"print","value":"0892-6638"},{"type":"electronic","value":"1530-6860"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}