{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T19:17:01Z","timestamp":1761679021673,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,6]],"date-time":"2006-03-06T00:00:00Z","timestamp":1141603200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:p>\n                    Dicer enzymes, found in nearly all eukaryotic cell types, cleave double\u2010stranded RNA substrates into the ~23 base\u2010pair products that are the effectors of gene silencing by RNA interference (RNAi). We describe the 3 \u00c5 crystal structure of the intact Dicer from\n                    <jats:italic>Giardia intestinalis<\/jats:italic>\n                    , revealing the structural basis for RNA recognition, measuring and cleavage by this class of enzymes. The hatchet\u2010shaped protein contains an RNA\u2010binding PAZ domain at one end and two RNase III domains at the other, connected by an N\u2010terminal domain and a long alpha\u2010helix. The connector helix sets the 60 \u00c5 distance between the PAZ and RNase III domains that corresponds the length of the double\u2010stranded RNA products of Dicer, implicating this structural element as the \u201cruler\u201d that defines interfering RNA size. Other details of the structure and biochemical activity of Dicer, as well as implications for its catalytic mechanism and interactions with ligands, will be discussed.\n                  <\/jats:p>","DOI":"10.1096\/fasebj.20.4.a423","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T16:29:46Z","timestamp":1624292986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Structural Basis for RNA Processing by Dicer"],"prefix":"10.1096","volume":"20","author":[{"given":"Jennifer A.","family":"Doudna","sequence":"first","affiliation":[{"name":"Molecular and Cell Biology UC Berkeley\/HHMI, 305 Hildebrand Hall Department of MCB University of California Berkeley CA 94720"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian J.","family":"MacRae","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology UC Berkeley\/HHMI, 305 Hildebrand Hall Department of MCB University of California Berkeley CA 94720"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Molecular and Cell Biology UC Berkeley\/HHMI, 305 Hildebrand Hall Department of MCB University of California Berkeley CA 94720"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,6]]},"container-title":["The FASEB Journal"],"original-title":[],"language":"en","deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:26:30Z","timestamp":1761675990000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fasebj.20.4.A423"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1096\/fasebj.20.4.A423"],"URL":"https:\/\/doi.org\/10.1096\/fasebj.20.4.a423","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"type":"print","value":"0892-6638"},{"type":"electronic","value":"1530-6860"}],"subject":[],"published":{"date-parts":[[2006,3]]},"assertion":[{"value":"2006-03-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}