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This variant has the ability to form fibrils in vitro under physiological conditions (PBS, pH 7.4). We studied by transmission electron microscopy the effect of the drug 4\u2032\u2010iodo\u20104\u2032\u2010deoxydoxorubicin (I\u2010DOX) on the in vitro assembly of TTR Leu55Pro fibrils by following fibril growth over a 15 day period. Our results showed that I\u2010DOX at a concentration of 10\n                    <jats:sup>\u22125<\/jats:sup>\n                    M\/100 \u00b5g fibrils does not inhibit fibril formation in up to 10 days since fibrils identical to the ones present in the untreated sample were observed. However, after 15 days of treatment, only round particles, resembling soluble native TTR, were observed. We also tested the ability of tetracyclines and nitrophenols to interfere with amyloid fibril formation for 17 days; the group of compounds tested showed fibril disruption activity to different extents: doxycycline and 2,4\u2010dinitrophenol resulted in complete disaggregation of fibrils. The species generated upon I\u2010DOX and tetracyclines treatments were nontoxic, as revealed by the lack of significant caspase\u20103 activation on a Schwannoma cell line, making them potential therapeutic drugs in TTR\u2010related and other amyloidosis.\u2014Cardoso, I., Merlini, G., Saraiva, M. J. 4\u2032\u2010iodo\u20104\u2032\u2010Deoxydoxorubicin and tetracyclines disrupt transthyretin amyloid fibrils in vitro producing noncytotoxic species: screening for TTR fibril disrupters.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    17, 803\u2013809 (2003)\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0764com","type":"journal-article","created":{"date-parts":[[2003,4,30]],"date-time":"2003-04-30T16:03:40Z","timestamp":1051718620000},"page":"803-809","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":108,"title":["4 \u2032\u2010iodo\u20104\u2032\u2010Deoxydoxorubicin and tetracyclines disrupt transthyretin amyloid fibrils in vitro producing noncytotoxic species: screening for TTR fibril disrupters"],"prefix":"10.1096","volume":"17","author":[{"given":"Isabel","family":"Cardoso","sequence":"first","affiliation":[{"name":"Amyloid Unit Institute for Molecular and Cell Biology University of Porto  Portugal"},{"name":"ICBAS University of Porto  Portugal"}]},{"given":"Giampaolo","family":"Merlini","sequence":"additional","affiliation":[{"name":"Biotechnology Research Laboratories University Hospital IRCCS Policlinico San Matteo Department of Biochemistry University of Pavia  Italy"}]},{"given":"Maria Jo\u00e3o","family":"Saraiva","sequence":"additional","affiliation":[{"name":"Amyloid Unit Institute for Molecular and Cell Biology University of Porto  Portugal"},{"name":"ICBAS University of Porto  Portugal"}]}],"member":"311","published-online":{"date-parts":[[2003,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/94.2.199"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(01)02480-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1006\/jmbi.2002.5441","article-title":"Transthyretin fibrillogenesis entails the assembly of monomers: a molecular model for in vitro assembled transthyretin amyloid-like fibrils","volume":"317","author":"Cardoso I.","year":"2002","journal-title":"J. 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