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Thus, we investigated the involvement of NPY receptors in mediating neuroprotection against excitotoxic insults in organotypic cultures of rat hippocampal slices. Exposure of hippocampal slice cultures to 2 \u03bcM AMPA (\u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u2010isoxazole\u20104\u2010propionate) induced neuronal degeneration, monitored by propidium iodide uptake, of granule cells and CA1 pyramidal cells. For dentate granule cells, selective activation of Y1, Y2, or Y5 receptors with 1 \u03bcM [Leu\n                    <jats:sup>31<\/jats:sup>\n                    ,Pro\n                    <jats:sup>34<\/jats:sup>\n                    ]NPY, 300 nM NPY13\u201336 or 1 \u03bcM 500 nM NPY(19\u201323)\u2010(Gly\n                    <jats:sup>1<\/jats:sup>\n                    ,Ser\n                    <jats:sup>3<\/jats:sup>\n                    ,Gln\n                    <jats:sup>4<\/jats:sup>\n                    ,Thr\n                    <jats:sup>6<\/jats:sup>\n                    ,Ala\n                    <jats:sup>31<\/jats:sup>\n                    ,Aib\n                    <jats:sup>32<\/jats:sup>\n                    ,Gln\n                    <jats:sup>34<\/jats:sup>\n                    )\u2010PP, respectively, had a neuroprotective effect against AMPA, whereas only the activation of Y2 receptors was effective for CA1 pyramidal cells. When the slice cultures were exposed to 6 \u03bcM kainate, the CA3 pyramidal cells displayed significant degeneration, and in this case the activation of Y1, Y2, and Y5 receptors was neuroprotective. For the kainic acid\u2010induced degeneration of CA1 pyramidal cells, it was again found that only the Y2 receptor activation was effective. Based on the present findings, it was concluded that Y1, Y2, and Y5 receptors effectively can modify glutamate receptor\u2010mediated neurodegeneration in the hippocampus.\n                  <\/jats:p>","DOI":"10.1096\/fj.02-0885fje","type":"journal-article","created":{"date-parts":[[2003,4,14]],"date-time":"2003-04-14T20:13:50Z","timestamp":1050351230000},"page":"1118-1120","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Activation of neuropeptide Y receptors is neuroprotective against excitotoxicity in organotypic hippocampal slice cultures"],"prefix":"10.1096","volume":"17","author":[{"given":"Ana P.","family":"Silva","sequence":"first","affiliation":[{"name":"Center for Neuroscience of Coimbra Department of Zoology University of Coimbra 3004-517 Coimbra Portugal"}]},{"given":"Paulo S.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Center for Neuroscience of Coimbra Department of Zoology University of Coimbra 3004-517 Coimbra Portugal"}]},{"given":"Ars\u00e9lio P.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Center for Neuroscience of Coimbra Department of Zoology University of Coimbra 3004-517 Coimbra Portugal"}]},{"given":"Caetana M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Center for Neuroscience of Coimbra Department of Zoology University of Coimbra 3004-517 Coimbra Portugal"}]},{"given":"Birthe","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology University of Southern Denmark - Odense DK-5000 Odense C Denmark"}]},{"given":"Jens","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Neurobiology University of Southern Denmark - Odense DK-5000 Odense C Denmark"}]},{"given":"Jo\u00e3o O.","family":"Malva","sequence":"additional","affiliation":[{"name":"Center for Neuroscience of Coimbra Department of Zoology University of Coimbra 3004-517 Coimbra Portugal"},{"name":"Institute of Biochemistry Faculty of Medicine University of Coimbra 3004-504 Coimbra Portugal"}]}],"member":"311","published-online":{"date-parts":[[2003,4,8]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"397","article-title":"Central nervous system pharmacology of neuropeptide Y. 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