{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T21:06:10Z","timestamp":1770325570890,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,4,20]],"date-time":"2012-04-20T00:00:00Z","timestamp":1334880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["10860"],"award-info":[{"award-number":["10860"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL-48129"],"award-info":[{"award-number":["HL-48129"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PIC\/IC\/83204\/2007"],"award-info":[{"award-number":["PIC\/IC\/83204\/2007"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The protein kinase liver kinase B1 (LKB1) regulates cell polarity and intercellular junction stability. Also, LKB1 controls the activity of salt\u2010inducible kinase 1 (SIK1). The role and relevance of SIK1 and its downstream effectors in linking the LKB1 signals within these processes are partially understood. We hypothesize that SIK1 may link LKB1 signals to the maintenance of epithelial junction stability by regulating E\u2010cadherin expression. Results from our studies using a mouse lung alveolar epithelial (MLE\u201012) cell line or human renal proximal tubule (HK2) cell line transiently or stably lacking the expression of SIK1 (using SIK1 siRNAs or shRNAs), or with its expression abrogated (\n                    <jats:italic>\n                      sik1\n                      <jats:sup>+\/+<\/jats:sup>\n                      vs. sik1\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice), indicate that suppression of SIK1 (\u223c40%) increases the expression of the transcriptional repressors Snail2 (\u223c12\u2010fold), Zeb1 (\u223c100%), Zeb2 (\u223c50%), and TWIST (\u223c20\u2010fold) by activating cAMP\u2010response element binding protein. The lack of SIK1 and activation of transcriptional repressors decreases the availability of E\u2010cadherin (mRNA and protein expression by \u223c100 and 80%, respectively) and the stability of intercellular junctions in epithelia (decreases in transepithelial resistance). Furthermore, LKB1\u2010mediated increases in E\u2010cadherin expression are impaired in cells where SIK1 has been disabled. We conclude that SIK1 is a key regulator of E\u2010cadherin expression, and thereby contributes to the stability of intercellular junctions.\u2014Eneling, K., Brion, L., Pinto, V., Pinho, M. J., Sznajder, J. I., Mochizuki, N., Emoto, K., Soares\u2010da\u2010Silva, P., Bertorello, A. M. Salt\u2010inducible kinase 1 regulates E\u2010cadherin expression and intercellular junction stability.\n                    <jats:italic>FASEB J.<\/jats:italic>\n                    26, 3230\u20133239 (2012).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.12-205609","type":"journal-article","created":{"date-parts":[[2012,4,21]],"date-time":"2012-04-21T02:59:12Z","timestamp":1334977152000},"page":"3230-3239","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Salt\u2010inducible kinase 1 regulates E\u2010cadherin expression and intercellular junction stability"],"prefix":"10.1096","volume":"26","author":[{"given":"Kristina","family":"Eneling","sequence":"first","affiliation":[{"name":"Membrane Signaling Networks, Atherosclerosis Research Unit, Department of Medicine, Karolinska Institutet, Center for Molecular Medicine Karolinska University Hospital\u2010Solna  Stockholm Sweden"}]},{"given":"Laura","family":"Brion","sequence":"additional","affiliation":[{"name":"Membrane Signaling Networks, Atherosclerosis Research Unit, Department of Medicine, Karolinska Institutet, Center for Molecular Medicine Karolinska University Hospital\u2010Solna  Stockholm Sweden"}]},{"given":"Vanda","family":"Pinto","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Faculty of Medicine  Porto Portugal"}]},{"given":"Maria J.","family":"Pinho","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Faculty of Medicine  Porto Portugal"}]},{"given":"Jacob I.","family":"Sznajder","sequence":"additional","affiliation":[{"name":"Department of Medicine Northwestern University  Chicago Illinois USA"}]},{"given":"Naoki","family":"Mochizuki","sequence":"additional","affiliation":[{"name":"Department of Cell Biology National Cerebral and Cardiovascular Center  Osaka Japan"}]},{"given":"Kazuo","family":"Emoto","sequence":"additional","affiliation":[{"name":"Osaka Bioscience Institute  Osaka Japan"}]},{"given":"Patricio","family":"Soares\u2010da\u2010Silva","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Faculty of Medicine  Porto Portugal"}]},{"given":"Alejandro M.","family":"Bertorello","sequence":"additional","affiliation":[{"name":"Membrane Signaling Networks, Atherosclerosis Research Unit, Department of Medicine, Karolinska Institutet, Center for Molecular Medicine Karolinska University Hospital\u2010Solna  Stockholm Sweden"}]}],"member":"311","published-online":{"date-parts":[[2012,4,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600110"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.01.042"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05291.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03967"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00708-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(94)90056-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009029","article-title":"Lack of sik1 in mouse embryonic stem cells impairs cardiomyogenesis by down\u2010regulating the cyclindependent kinase inhibitor p57kip2","volume":"5","author":"Romito A.","year":"2010","journal-title":"PLoS One"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706838104"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","DOI":"10.1126\/scisignal.2000369","article-title":"SIK1 couples LKB1 to p53\u2010dependent anoikis and suppresses metastasis","volume":"2","author":"Cheng H.","year":"2009","journal-title":"Sci. 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