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Sixty\u2010eight genes involved in lipid metabolism were changed in MEC differentiation. Differentiated cells showed induction of\n                    <jats:italic>Elovl6<\/jats:italic>\n                    (2\u2010fold),\n                    <jats:italic>Scd1<\/jats:italic>\n                    (4\u2010fold), and\n                    <jats:italic>Fads2<\/jats:italic>\n                    (2\u2010fold), which correlated with increased levels of C16:1\n                    <jats:italic>n<\/jats:italic>\n                    \u20137 and C18:1\n                    <jats:italic>n<\/jats:italic>\n                    \u20139 (1.5\u2010fold), C20:3\n                    <jats:italic>n<\/jats:italic>\n                    \u20136 (2.5\u2010fold), and C20:4\n                    <jats:italic>n<\/jats:italic>\n                    \u20136 (6\u2010fold) fatty acids (FAs) and more phospholipids (PLs) containing these species. Further, increased expression (2\u2010 to 3\u2010fold) of genes in phosphatidylethanolamine (PE)\n                    <jats:italic>de novo<\/jats:italic>\n                    biosynthesis resulted in a 20% PE increase. Proliferating\/ undifferentiated cells showed higher C16:0 (1.7\u2010fold) and C18:2\n                    <jats:italic>n<\/jats:italic>\n                    \u20136 (4.2\u2010fold) levels and more PLs containing C16:0 FAs [PC(16:0\/16:1), PG(16:0\/18:2), PG(16: 0\/18:1), and SM(16:0\/18:0)]. Kaplan\u2010Meier analysis of data from 3455 patients with breast cancer disclosed a positive correlation for 59% of genes expressed in differentiated MECs with better survival. PE biosynthesis and FA oxidation correlated with better prognosis in patients with breast cancer, including the basal\u2010like subtype. Therefore, genes involved in mammary gland FA and PL metabolism and their resulting molecular species reflect the cellular proliferative ability and differentiation state and deserve further studies as potential markers of breast cancer progression.\u2014 D\u00f3ria, M. L., Ribeiro, A. S., Wang, J., Cotrim, C. Z., Domingues, P., Williams, C., Domingues, M. R., Helguero, L. A., Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival. FASEB J. 28, 4247\u20134264 (2014).\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.fasebj.org\">www.fasebj.org<\/jats:ext-link>\n                  <\/jats:p>","DOI":"10.1096\/fj.14-249672","type":"journal-article","created":{"date-parts":[[2014,6,27]],"date-time":"2014-06-27T00:02:29Z","timestamp":1403827349000},"page":"4247-4264","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Fatty acid and phospholipid biosynthetic pathways are regulated throughout mammary epithelial cell differentiation and correlate to breast cancer survival"],"prefix":"10.1096","volume":"28","author":[{"given":"M. Luisa","family":"D\u00f3ria","sequence":"first","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]},{"given":"Ana S.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biology and Biochemistry Center for Nuclear Receptors and Cell Signaling University of Houston Houston Texas USA"}]},{"given":"C\u00e2ndida Z.","family":"Cotrim","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]},{"given":"Pedro","family":"Domingues","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]},{"given":"Cecilia","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Biology and Biochemistry Center for Nuclear Receptors and Cell Signaling University of Houston Houston Texas USA"}]},{"given":"M. Ros\u00e1rio","family":"Domingues","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]},{"given":"Luisa A.","family":"Helguero","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Centre Organic Chemistry and Natural Products Research Unit Department of Chemistry Universidade de Aveiro Campus de Santiago Aveiro Portugal"}]}],"member":"311","published-online":{"date-parts":[[2014,6,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-014-2852-7"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09781"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"a013417","DOI":"10.1101\/cshperspect.a013417","article-title":"On chromatin remodeling in mammary gland differentiation and breast tumorigenesis","volume":"4","author":"Polyak K.","year":"2012","journal-title":"Cold Spring Harb. 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