{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T02:16:44Z","timestamp":1774405004601,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T00:00:00Z","timestamp":1625875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI\u201001\u20100145\u2010FEDER\u2010028656"],"award-info":[{"award-number":["POCI\u201001\u20100145\u2010FEDER\u2010028656"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/04539\/2020"],"award-info":[{"award-number":["UIDB\/04539\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/04539\/2020"],"award-info":[{"award-number":["UIDP\/04539\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BPD\/115546\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/115546\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PD\/BD\/135498\/2018"],"award-info":[{"award-number":["PD\/BD\/135498\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["IF\/01302\/2012"],"award-info":[{"award-number":["IF\/01302\/2012"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["faseb.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The FASEB Journal"],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The synaptic expression of glutamate receptors of the \u03b1\u2010amino\u20103\u2010hydroxy\u20105\u2010methylisoxazole\u20104\u2010propionic acid (AMPA) type is dynamically controlled by interaction with binding partners and auxiliary proteins. These proteins can be regulated by posttranslational modifications, including ubiquitination. In this work, we investigated the regulation of glutamate receptor interacting protein\u2010associated protein 1 (GRASP1) by ubiquitin\u2010dependent mechanisms and its impact on surface expression and activity of synaptic AMPA receptors. Cotransfection of GFP\u2010ubiquitin decreased myc\u2010GRASP1 protein levels in HEK293T cells, and this effect was inhibited upon transfection of an ubiquitin mutant that cannot be ubiquitinated on Lys48. In addition, transfection of cultured hippocampal neurons with GFP\u2010ubiquitin reduced the dendritic levels of endogenous GRASP1 and decreased the surface expression of GluA1 AMPA receptor subunits, an effect that was partly reversed by cotransfection with GRASP1. Similarly, transfection of hippocampal neurons with GFP\u2010ubiquitin decreased the amplitude of miniature excitatory postsynaptic currents (mEPSCs) mediated by Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u2010impermeable AMPA receptors, and this effect was abrogated by cotransfection of GRASP1. Together, the results show a role for ubiquitination in the regulation of the postsynaptic protein GRASP1, which has an impact on the surface distribution of AMPA receptors and on their activity at the synapse.\n                  <\/jats:p>","DOI":"10.1096\/fj.201903061rr","type":"journal-article","created":{"date-parts":[[2021,7,10]],"date-time":"2021-07-10T13:26:57Z","timestamp":1625923617000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["GRASP1 ubiquitination regulates AMPA receptor surface expression and synaptic activity in cultured hippocampal neurons"],"prefix":"10.1096","volume":"35","author":[{"given":"Miranda","family":"Mele","sequence":"first","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}]},{"given":"Pasqualino","family":"De Luca","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}]},{"given":"Ana Rita","family":"Santos","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"}]},{"given":"Marta","family":"Vieira","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"}]},{"given":"Ivan L.","family":"Salazar","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}]},{"given":"Paulo S.","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"Institute for Interdisciplinary Research University of Coimbra Coimbra Portugal"}]},{"given":"Carlos B.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"},{"name":"Department of Life Sciences University of Coimbra Coimbra Portugal"}]}],"member":"311","published-online":{"date-parts":[[2021,7,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.10.018"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00391"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00361"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2011.09.008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.10.025"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP278701"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP276698"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aar6889"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000283"},{"issue":"1405","key":"e_1_2_9_11_1","first-page":"e1408","article-title":"GRASP1 regulates synaptic plasticity and learning through endosomal recycling of AMPA receptors","volume":"93","author":"Chiu SL","year":"2017","journal-title":"Neuron"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81198-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.10.073"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.01.015"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.10.011"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2452-14.2014"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13271"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2017.00347"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1013"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2014.08.006"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0218-19.2020"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-015-1752-5"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.356"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aav3577"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr300536k"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109023200"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200510071"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4474-04.2005"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.16-06-01982.1996"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-0102(97)00067-9"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4521-14.2015"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1101\/lm.984508"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3291-13.2014"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2017.09.002"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14630"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5944-10.2011"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2011.06.008"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2011.12.006"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04769"}],"container-title":["The FASEB Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201903061RR","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1096\/fj.201903061RR","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/pdf\/10.1096\/fj.201903061RR","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:58:37Z","timestamp":1761699517000},"score":1,"resource":{"primary":{"URL":"https:\/\/faseb.onlinelibrary.wiley.com\/doi\/10.1096\/fj.201903061RR"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,10]]},"references-count":38,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2021,8]]}},"alternative-id":["10.1096\/fj.201903061RR"],"URL":"https:\/\/doi.org\/10.1096\/fj.201903061rr","archive":["Portico"],"relation":{},"ISSN":["0892-6638","1530-6860"],"issn-type":[{"value":"0892-6638","type":"print"},{"value":"1530-6860","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,10]]},"assertion":[{"value":"2019-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e21763"}}