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We have previously demonstrated that infection of dendritic cells (DC) by\n                    <jats:italic>Leishmania amazonensis<\/jats:italic>\n                    leads to increased expression of CD39 and CD73 and to the selective activation of the low affinity A\n                    <jats:sub>2B<\/jats:sub>\n                    receptors (A\n                    <jats:sub>2B<\/jats:sub>\n                    R), which contributes to DC inhibition, without involvement of the high affinity A\n                    <jats:sub>2A<\/jats:sub>\n                    R. To understand this apparent paradox, we now characterized the alterations of both adenosine receptors in infected cells. With this aim, bone marrow\u2010derived DC from C57BL\/6J mice were infected with metacyclic promastigotes of\n                    <jats:italic>L. amazonensis<\/jats:italic>\n                    . Fluorescence microscopy revealed that\n                    <jats:italic>L. amazonensis<\/jats:italic>\n                    infection stimulates the recruitment of A\n                    <jats:sub>2B<\/jats:sub>\n                    R, but not of A\n                    <jats:sub>2A<\/jats:sub>\n                    R, to the surface of infected DC, without altering the amount of mRNA or the total A\n                    <jats:sub>2B<\/jats:sub>\n                    R density, an effect dependent on lipophosphoglycan (LPG). Log\u2010phase promastigotes or axenic amastigotes of\n                    <jats:italic>L. amazonensis<\/jats:italic>\n                    do not stimulate A\n                    <jats:sub>2B<\/jats:sub>\n                    R recruitment. A\n                    <jats:sub>2B<\/jats:sub>\n                    R clusters are localized in caveolin\u2010rich lipid rafts and the disruption of these membrane domains impairs A\n                    <jats:sub>2B<\/jats:sub>\n                    R recruitment and activation. More importantly, our results show that A\n                    <jats:sub>2B<\/jats:sub>\n                    R co\u2010localize with CD39 and CD73 forming a \u201cpurinergic cluster\u201d that allows for the production of extracellular adenosine in close proximity with these receptors. We conclude that A\n                    <jats:sub>2B<\/jats:sub>\n                    R activation by locally produced adenosine constitutes an elegant and powerful evasion mechanism used by\n                    <jats:italic>L. amazonensis<\/jats:italic>\n                    to down\u2010modulate the DC activation.\n                  <\/jats:p>","DOI":"10.1096\/fj.202002396rr","type":"journal-article","created":{"date-parts":[[2021,4,4]],"date-time":"2021-04-04T05:57:35Z","timestamp":1617515855000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Clustering of adenosine A\n                    <sub>2B<\/sub>\n                    receptors with ectonucleotidases in caveolin\u2010rich lipid rafts underlies immunomodulation by\n                    <i>Leishmania amazonensis<\/i>"],"prefix":"10.1096","volume":"35","author":[{"given":"Amanda Braga","family":"Figueiredo","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas Universidade Federal de Ouro Preto Ouro Preto Brazil"}]},{"given":"Renata Alves","family":"de Oliveira e Castro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Imunoparasitologia N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas Universidade Federal de Ouro Preto Ouro Preto Brazil"}]},{"given":"N\u00edvia Carolina","family":"Nogueira\u2010Paiva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Multiusu\u00e1rio de Microscopia N\u00facleo de Pesquisas em Ci\u00eancias Biol\u00f3gicas Universidade Federal de Ouro Preto Ouro Preto Brazil"}]},{"given":"Filipa","family":"Moreira","sequence":"additional","affiliation":[{"name":"CNC\u2010Center for Neuroscience and Cell Biology University of Coimbra Coimbra Portugal"}]},{"given":"Francisco 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