{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:22:39Z","timestamp":1773102159682,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,2]],"date-time":"2009-01-02T00:00:00Z","timestamp":1230854400000},"content-version":"vor","delay-in-days":701,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Laryngoscope"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives:<\/jats:bold> Replication\u2010competent, vesicular stomatitis virus (VSV) has been demonstrated to be an effective oncolytic agent in a variety of malignant tumors. Cytokine gene transfer has also been used as immunomodulatory therapy for cancer. To test the use of combining these two approaches, an oncolytic VSV vector (rVSV\u2010IL12) was designed to express the murine interleukin 12 (IL12) gene. This cytokine\u2010carrying oncolytic virus was compared with an analogous noncytokine\u2010carrying fusogenic virus (rVSV\u2010F) in the treatment of murine SCC VII squamous cell carcinoma (SCC).<\/jats:p><jats:p><jats:bold>Study Design and Setting:<\/jats:bold> The authors performed in vitro testing of recombinant VSV\u2010F and recombinant VSV\u2010IL12 in SCC cell lines. In vivo testing of multiple direct intratumoral injections of rVSV\u2010F or rVSV\u2010IL12 in an orthotopic floor of mouth murine model was performed. Each cell line was tested using rVSV\u2010F or rVSV\u2010IL12 at multiplicity of infection of 0.01. The ability of each virus to replicate was tested by real\u2010time reverse transcriptase\u2013polymerase chain reaction over 48 hours to determine viral copies of RNA. Cell survival was determined by MTT assay over 72 hours. IL12 expression by rVSV\u2010IL12\u2010treated cells was determined by enzyme\u2010linked immunosorbent assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Both viruses demonstrated similar infection efficiency, viral replication, and cytotoxicity in vitro. In an SCC VII orthotopic floor of mouth model in immunocompetent C3H\/HeJ mice, multiple intratumoral injections with each virus caused a significant reduction in tumor volume when compared with saline injections alone. The rVSV\u2010IL12\u2010treated tumors showed a striking reduction in tumor volume when compared with rVSV\u2010F and saline\u2010treated tumors (<jats:italic>P<\/jats:italic> &lt; .005). This striking reduction in tumor volume translated into a substantial survival benefit in rVSV\u2010IL12\u2010treated animals. No treatment\u2010related toxicity was observed in either group.<\/jats:p><jats:p><jats:bold>Conclusion\/Significance:<\/jats:bold> rVSV\u2010IL12 is a novel oncolytic vesicular stomatitis virus that effectively expresses IL12 and significantly enhances the treatment of head and neck murine carcinoma. Such combined oncolytic and immunomodulatory strategies hold promise in the treatment of head and neck cancers.<\/jats:p>","DOI":"10.1097\/01.mlg.0000246194.66295.d8","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T18:33:28Z","timestamp":1167935608000},"page":"210-214","source":"Crossref","is-referenced-by-count":56,"title":["Interleukin\u201012 Expression Enhances Vesicular Stomatitis Virus Oncolytic Therapy in Murine Squamous Cell Carcinoma"],"prefix":"10.1002","volume":"117","author":[{"given":"Edward J.","family":"Shin","sequence":"first","affiliation":[]},{"given":"Georges B.","family":"Wanna","sequence":"additional","affiliation":[]},{"given":"Bryan","family":"Choi","sequence":"additional","affiliation":[]},{"suffix":"III","given":"Demetrio","family":"Aguila","sequence":"additional","affiliation":[]},{"given":"Oliver","family":"Ebert","sequence":"additional","affiliation":[]},{"given":"Eric M.","family":"Genden","sequence":"additional","affiliation":[]},{"given":"Savio L.","family":"Woo","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2009,1,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.50.1.7"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1992.10.2.257"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200003001-00001"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209042"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3753"},{"key":"e_1_2_7_7_2","doi-asserted-by":"crossref","first-page":"5465","DOI":"10.4049\/jimmunol.160.11.5465","article-title":"T cell\u2010 and NK cell independent inhibition of hepatic metastases by systemic administration of an IL\u201012\u2010expressing recombinant adenovirus.","volume":"160","author":"Siders WM","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300550"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990531)81:5<813::AID-IJC24>3.0.CO;2-I"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.1-1-88"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-2494(96)83041-0"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.21.11302"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1997.01900010022003"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20536"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.4.1223"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0081"}],"container-title":["The Laryngoscope"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1097%2F01.mlg.0000246194.66295.d8","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1097\/01.mlg.0000246194.66295.d8","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T04:19:28Z","timestamp":1695961168000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1097\/01.mlg.0000246194.66295.d8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,2]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,2]]}},"alternative-id":["10.1097\/01.mlg.0000246194.66295.d8"],"URL":"https:\/\/doi.org\/10.1097\/01.mlg.0000246194.66295.d8","archive":["Portico"],"relation":{},"ISSN":["0023-852X","1531-4995"],"issn-type":[{"value":"0023-852X","type":"print"},{"value":"1531-4995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,2]]}}}