{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T07:19:19Z","timestamp":1777792759209,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cereb Blood Flow Metab"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:p>HDJ-2, a member of the HSP40 family, functions as a cochaperone to promote protein folding both by binding to unfolded polypeptides and by regulating the activity of HSP70. This study tested whether HDJ-2 overexpression alone could provide significant protection from ischemia-like injury. Primary mouse astrocyte cultures were infected with an HDJ-2 encoding retroviral vector or control retrovirus lacking HDJ-2. Expression of HDJ-2 was confirmed by immunohistochemical staining and immunoblotting. Injury paradigms to mimic ischemia were glucose deprivation (GD) for 24 hours and oxygen-glucose deprivation (OGD) for 8 hours. Cell death was determined by trypan blue exclusion and cell counting. Overexpression of HDJ-2 alone significantly reduced astrocyte injury after both GD and OGD, independent of an elevation in HSP70. To further search for the mechanism of HDJ-2 protection, cultured astrocytes allowed to recover 16 hours after 8 hours GD were labeled with a monoclonal antiubiquitin antibody that recognizes both free ubiquitin and ubiquitinated proteins. The immunolabeling pattern changed from a relatively even distribution in both nuclei and cytoplasm in control cells to heterogeneous aggregates and marked reduction of nuclear staining in most of the cells after GD. When HDJ-2 was overexpressed, the number of cells with evidence of protein aggregation was significantly reduced. Thus, blocking protein aggregation may be an important mechanism by which HDJ-2 protects cells from damage.<\/jats:p>","DOI":"10.1097\/01.wcb.0000088765.02615.fe","type":"journal-article","created":{"date-parts":[[2003,11,18]],"date-time":"2003-11-18T09:35:34Z","timestamp":1069148134000},"page":"1113-1116","source":"Crossref","is-referenced-by-count":17,"title":["Overexpression of HDJ-2 Protects Astrocytes from Ischemia-Like Injury and Reduces Redistribution of Ubiquitin Staining\n                    <i>in Vitro<\/i>"],"prefix":"10.1177","volume":"23","author":[{"given":"Yanli","family":"Qiao","sequence":"first","affiliation":[{"name":"From the Department of Anesthesia, Stanford University School of Medicine, Stanford, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Bing","family":"Ouyang","sequence":"additional","affiliation":[{"name":"From the Department of Anesthesia, Stanford University School of Medicine, Stanford, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rona G","family":"Giffard","sequence":"additional","affiliation":[{"name":"From the Department of Anesthesia, Stanford University School of Medicine, Stanford, California, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2003,10,1]]},"reference":[{"key":"bibr1-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01276-4"},{"key":"bibr2-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-23-10338.1999"},{"key":"bibr3-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.2990"},{"key":"bibr4-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-06-04545.1995"},{"key":"bibr5-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01267-2"},{"key":"bibr6-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1038\/381571a0"},{"key":"bibr7-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.425"},{"key":"bibr8-01.WCB.0000088765.02615.FE","doi-asserted-by":"crossref","first-page":"3191","DOI":"10.1523\/JNEUROSCI.20-09-03191.2000","volume":"20","author":"Hu BR","year":"2000","journal-title":"J Neursoci"},{"key":"bibr9-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200107000-00012"},{"key":"bibr10-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.13.2009"},{"key":"bibr11-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9525(98)01559-5"},{"key":"bibr12-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.8.4239"},{"key":"bibr13-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.32.19617"},{"key":"bibr14-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1080\/026567300285259"},{"key":"bibr15-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5849(02)00912-7"},{"key":"bibr16-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1097\/01.WCB.0000055774.06337.F6"},{"key":"bibr17-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200011000-00004"},{"key":"bibr18-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(00)01721-3"},{"key":"bibr19-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199601310-00013"},{"key":"bibr20-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1379\/1466-1268(1997)002<0162:TMETHI>2.3.CO;2"},{"key":"bibr21-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.5.731"},{"key":"bibr22-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.8.5.795"},{"key":"bibr23-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)13444-9"},{"key":"bibr24-01.WCB.0000088765.02615.FE","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440403"}],"container-title":["Journal of Cerebral Blood Flow &amp; Metabolism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1097\/01.WCB.0000088765.02615.FE","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1097\/01.WCB.0000088765.02615.FE","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1097\/01.WCB.0000088765.02615.FE","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T20:07:57Z","timestamp":1777493277000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1097\/01.WCB.0000088765.02615.FE"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2003,10]]}},"alternative-id":["10.1097\/01.WCB.0000088765.02615.FE"],"URL":"https:\/\/doi.org\/10.1097\/01.wcb.0000088765.02615.fe","relation":{},"ISSN":["0271-678X","1559-7016"],"issn-type":[{"value":"0271-678X","type":"print"},{"value":"1559-7016","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10]]}}}